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目的:经对不同提取工艺制备的羊耳菊提取物进行比较研究,确定合理可行的提取工艺。方法:选用主要药效学试验结合指标成分收率和指纹图谱变化,对方中羊耳菊的水煎煮提取物、水提醇沉物及树脂分离精制纯化的样品进行比较,以评价羊耳菊活性成分的提取工艺。结果:羊耳菊水煮、醇沉、大孔吸附树脂富集纯化各样品的抗炎、解热、抗菌作用无显著性差异,HPLC图谱显示经大孔吸附树脂富集纯化后样品杂质峰减少,3,4-O-二咖啡酰基-奎宁酸转移率均达70%以上。结论:大孔树脂纯化羊耳菊活性成分的工艺合理可行,该工艺可最大限度富集有效成分。
OBJECTIVE: To compare and research the extracts of Chrysanthemum morifolium prepared by different extraction techniques to determine a reasonable and feasible extraction process. Methods: The main pharmacodynamics test was used to determine the yield of components and changes in fingerprints. The decoction of water extracts, water extraction and alcohol precipitation, and the separation and purification of purified resin from each other were compared to evaluate Chinchilla. Active ingredient extraction process. Results: There was no significant difference in anti-inflammatory, antipyretic and antibacterial activities of the samples collected from boiled, alcohol-sinked, and macroporous resin. The HPLC spectra showed that the impurity peaks of the sample after enrichment and purification with macroporous adsorption resin decreased. The transfer rate of 3,4-O-dicaffeoyl-quinic acid reached over 70%. Conclusion: The process of purification of active constituents of Chrysanthemum by macroporous resin is reasonable and feasible. This process can maximize the enrichment of active ingredients.