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本文探讨了葛根素对牛内皮细胞的作用。在细胞培养液中加入葛根素(250μg/ml)为实验组;不加则为对照组。经37℃6h 培养后,分别测定内皮细胞内外液中羟(?)氨酸和糖胺多糖的含量。结果表明,葛根素对牛内皮细胞有明显作用,羟脯氨酸含量由对照组细胞内液和外液中的3.35±0.05μg/ml 和2.68±0.10μg/ml 下降至实验组的2.70±0.06μg/ml(P<0.01)和2.43±0.21μg/ml(P<0.05);糖胺多糖由对照组细胞内液和外液的1.17±0.12μg/ml 和1.57±0.08μg/ml,降至实验组的0.95±0.12μg/ml(P<0.01)和1.68±0.15/μg/ml。
This article explored the effect of puerarin on bovine endothelial cells. Puerarin (250 μg/ml) was added to the cell culture medium as the experimental group; if not, it was the control group. After culturing at 37°C for 6 hours, the contents of hydroxyproline and glycosaminoglycan in the inner and outer fluids of endothelial cells were measured. The results showed that puerarin had significant effect on bovine endothelial cells. The content of hydroxyproline was decreased from 3.35±0.05μg/ml and 2.68±0.10μg/ml in the intracellular fluid and the external fluid of the control group to 2.70±0.06 in the experimental group. Μg/ml (P<0.01) and 2.43±0.21 μg/ml (P<0.05); glycosaminoglycans decreased from 1.17±0.12 μg/ml and 1.57±0.08 μg/ml in the intracellular and extracellular fluids of the control group. The experimental group 0.95±0.12 μg/ml (P<0.01) and 1.68±0.15/μg/ml.