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目的以兔VX2肝癌模型为对象,探讨经导管动脉注入脂质体介导的p53基因治疗肝癌的可行性及转染和表达情况。方法将pCMV-myc-p53质粒、阳离子脂质体LipofectAMINE以及pCMV-myc- p53和LipofectAMINE的复合体分别注入兔VX2肝癌模型的肿瘤供血动脉,并提取肿瘤组织蛋白,采用蛋白印迹法及免疫组化检测基因转染及其表达。以不同量的pCMV-myc-p53与LipofectAMINE形成的复合体分别注入兔VX2肝癌模型的肿瘤供血动脉内,同法检测基因的转染及其表达。结果脂质体介导的p53基因经动脉途径成功转染了兔VX2肝癌模型的肿瘤组织并进行表达,其转染效率明显高于单纯基因导入,基因的量与转染效率之间存在量效关系。结论经动脉途径导入脂质体介导的p53基因治疗肝癌是可行、有效的,具有广阔的应用前景。
Objective To investigate the feasibility and transfection and expression of transfection of liposome-mediated p53 gene into hepatocellular carcinoma (HCC) by rabbit VX2 liver cancer model. Methods The complex of pCMV-myc-p53 plasmid, cationic liposome LipofectAMINE, pCMV-myc-p53 and LipofectAMINE was injected into the tumor-supplying artery of rabbit VX2 hepatocellular carcinoma model and the tumor tissue protein was extracted. Western blotting and immunohistochemistry Detection of gene transfection and its expression. The complex of pCMV-myc-p53 and LipofectAMINE was injected into the tumor-supplying artery of rabbit VX2 hepatic carcinoma model respectively. The transfection and expression of the gene were detected by the same method. Results The liposome-mediated p53 gene was successfully transfected into the tumor tissue of VX2 rabbit model by arterial route and its transfection efficiency was significantly higher than that of simple gene transfection. There was a quantitative effect between the amount of gene and the transfection efficiency relationship. Conclusion It is feasible and effective to introduce liposome-mediated p53 gene into liver cancer via arterial approach and has broad application prospects.