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目的:观察实验性自身免疫性脑脊髓炎(EAE)发病高峰期淋巴细胞表面B7-H1分子的表达变化,探讨B7-H1在EAE发病和病情进展中的作用。方法:以MOG35-55诱导雌性C58BL/6小鼠制备EAE动物模型,并对其行为学变化进行记录与评分。分别于发病前和发病高峰期处死小鼠,分离脾脏与中枢神经系统中的淋巴细胞,用流式细胞术分析淋巴细胞表面B7-H1分子的表达差异。结果:与发病前相比,EAE发病高峰期脾脏分离的淋巴细胞中B7-H1阳性细胞率由(35.1±2.46)%下降至(18.9±2.06)%(P<0.01),其中CD4+T细胞中B7-H1阳性细胞由发病前的(6.4±1.25)%下降至发病后的(2.3±1.04)%(P<0.05)。中枢神经系统浸润的淋巴细胞中有8.7%为B7-H1阳性细胞。结论:在EAE发病过程中,B7-H1分子在外周淋巴细胞的阳性细胞数下降,而中枢神经系统浸润的淋巴细胞中有一定比例的B7-H1阳性细胞,提示B7-H1可能在EAE发病过程中发挥重要作用。
OBJECTIVE: To observe the expression of B7-H1 on the surface of lymphocytes at the peak of experimental autoimmune encephalomyelitis (EAE) and to explore the role of B7-H1 in the pathogenesis and progression of EAE. Methods: Female C58BL / 6 mice were induced by MOG35-55 to prepare animal models of EAE. The behavioral changes were recorded and scored. Mice were sacrificed before and at the peak of onset, lymphocytes were isolated from spleen and central nervous system, and the expression of B7-H1 on the surface of lymphocytes was analyzed by flow cytometry. Results: Compared with those before onset, the rate of B7-H1 positive cells in spleen lymphocytes decreased from (35.1 ± 2.46)% to (18.9 ± 2.06)% (P <0.01) at the peak of EAE. CD4 + T cells The B7-H1 positive cells decreased from (6.4 ± 1.25)% before onset to (2.3 ± 1.04)% after onset (P <0.05). 8.7% of CNS infiltrating lymphocytes were B7-H1 positive cells. Conclusions: During the process of EAE, the number of positive cells of B7-H1 in peripheral lymphocytes decreased while the proportion of B7-H1 positive cells in central nervous system infiltrating lymphocytes suggested that B7-H1 may play an important role in the pathogenesis of EAE In the play an important role.