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目的:探讨胰腺癌患者化疗前后外周血中自然杀伤T细胞(NKT)、T淋巴细胞亚群及γ干扰素等细胞因子的变化及其意义。方法:将确诊的42例胰腺癌实施化疗的患者作为胰腺癌组,另选30例消化内科收治的胰腺炎患者为胰腺炎组、30例体检健康人群为健康组,比较三组外周血中自然杀伤T细胞、T淋巴细胞亚群及γ干扰素等细胞因子水平差异。结果:胰腺癌组在化疗前IFN-γ、肿瘤坏死因子ɑ(TNF-ɑ)、白细胞介素2(IL-2)水平分别显著的低于化疗后(P<0.05);胰腺癌组患者化疗前的白细胞介素6(IL-6)显著的高于化疗后(P<0.05);胰腺癌组患者化疗前、化疗后的IFN-γ、TNF-ɑ、IL-2水平均显著的低于健康组和胰腺炎组,胰腺癌组化疗前后IL-6水平均显著的高于健康组和胰腺炎组(均P<0.05);胰腺癌组在化疗前NKT细胞显著的低于化疗后(P<0.05);胰腺癌组患者化疗前、化疗后的NKT细胞均显著的低于健康组和胰腺炎组(P<0.05);胰腺癌组患者在化疗前T淋巴细胞(CD3+、CD4+、CD4+/CD8+)水平均显著的低于化疗后(P<0.05),胰腺癌组患者化疗前的CD8+显著的高于化疗后(P<0.05)。结论:胰腺癌患者外周血免疫相关细胞及细胞因子水平较健康人群显著的降低,化疗后有助于改善患者的免疫水平、在化疗过程中检测免疫细胞及细胞因子变化可以对患者的化疗效果进行评价。
Objective: To investigate the changes and their significance of natural killer T cells (NKT), T lymphocyte subsets and interferon - γ in peripheral blood of patients with pancreatic cancer before and after chemotherapy. Methods: Forty-two patients with pancreatic cancer undergoing chemotherapy were selected as pancreatic cancer group, another 30 patients with pancreatitis admitted to the Department of Gastroenterology were selected as pancreatitis group, 30 healthy people as healthy group, and three groups of peripheral blood natural Killer T cells, T lymphocyte subsets and interferon gamma and other cytokines levels. Results: The levels of IFN-γ, TNF-ɑ and IL-2 in patients with pancreatic cancer before chemotherapy were significantly lower than those after chemotherapy (P <0.05) The levels of IL-6 before chemotherapy were significantly higher than those after chemotherapy (P <0.05). The levels of IFN-γ, TNF-ɑ and IL-2 in patients with pancreatic cancer before and after chemotherapy were significantly lower than those before chemotherapy The levels of IL-6 in healthy group, pancreatitis group and pancreatic cancer group before and after chemotherapy were significantly higher than those in healthy group and pancreatitis group (all P <0.05). NKT cells in pancreatic cancer group were significantly lower than those before chemotherapy (P <0.05). The numbers of NKT cells in the pancreatic cancer group before and after chemotherapy were significantly lower than those in the healthy group and the pancreatitis group (P <0.05). The levels of CD3 +, CD4 +, CD4 + CD8 +) were significantly lower than those after chemotherapy (P <0.05). The levels of CD8 + in patients with pancreatic cancer before chemotherapy were significantly higher than those after chemotherapy (P <0.05). Conclusion: The levels of peripheral blood-related immune cells and cytokines in patients with pancreatic cancer are significantly lower than those in healthy people. After chemotherapy, it is helpful to improve the immunological level of patients. Detecting changes of immune cells and cytokines in chemotherapy can be used to evaluate the effect of chemotherapy Evaluation.