论文部分内容阅读
目的:观察茶黄素对大鼠缺血性脑损伤所致炎症反应的作用。方法:64只健康成年SD大鼠随机分为4组:假手术组、模型组与茶黄素低(20 mg·kg-1)、高(40 mg·kg-1)剂量组。连续给药7 d后,除假手术组外,采用大脑中动脉线栓法(MCAO)制备大鼠实验性脑缺血模型。缺血24 h后,进行神经功能评分,然后每组随机抽取8只计算脑组织含水量,另外8只用于测定脑组织核因子-κB p65(NF-κB p65)mRNA水平。最后,测定血清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)及细胞间黏附分子-1(ICAM-1)含量。结果 :与假手术组相比,模型组大鼠神经功能评分、脑组织含水量、脑组织NF-κB p65 mRNA表达量、血清TNF-α、IL-1β及ICAM-1含量显著升高。与模型组相比,茶黄素高剂量组脑组织NF-κB p65 mRNA表达量明显减少,茶黄素低、高剂量组大鼠神经功能评分、脑组织含水量、血清TNF-α、IL-1β及ICAM-1含量显著降低。结论:茶黄素对大鼠缺血性脑损伤造成的炎症反应有一定的缓解作用。
Objective: To observe the effect of theaflavin on inflammatory reaction induced by ischemic brain injury in rats. Methods: Sixty-four healthy adult Sprague-Dawley rats were randomly divided into 4 groups: sham operation group, model group and low dose of theaflavin (20 mg · kg -1) and high dose (40 mg · kg -1). After continuous administration for 7 days, except the sham operation group, experimental middle cerebral artery occlusion (MCAO) was used to establish experimental cerebral ischemia model in rats. Neurological deficits were assessed 24 hours after ischemia. Then, eight mice were randomly selected to calculate the brain water content. The other eight were used to determine the level of NF-κB p65 mRNA. Finally, the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and intercellular adhesion molecule-1 (ICAM-1) were measured. Results: Compared with the sham operation group, the neurological function scores, the water content of brain tissue, the expression of NF-κB p65 mRNA, the levels of TNF-α, IL-1β and ICAM-1 in model group were significantly increased. Compared with the model group, the expression of NF-κB p65 mRNA in the high-dose group of theaflavin decreased significantly. The neurofunction scores, the water content of brain tissue, serum TNF-α, IL-1β, ICAM-1 content was significantly reduced. Conclusion: Theaflavin can relieve the inflammatory reaction induced by ischemic brain injury in rats.