本文采用鼠抗人IgG亚类单克隆抗体酶联免疫吸附试验测定小儿单纯性肾病综合征（INS）血清IgG亚类（18例）及血清肺炎球菌多糖（PnPs）抗体（10例），同时观察部分患PWM诱导的体外IgG亚类产生、BCGF_8、BCDF_3、IL-6活性及T、B细胞增殖功能，发现INS发作期儿童血清IgG亚类、PnPs特异性IgG亚类抗体、PWM诱导的体外IgG亚类均明显降低，缺陷类型相似，缺陷频度有沿IgCH基因5′-3′逐渐增高的趋势，IgG_3受累最少，而IgG_1、 In this study, serum IgG subclasses (18 cases) and serum pneumococcal polysaccharide (PnPs) antibodies (10 cases) were determined by mouse anti-human IgG subclass monoclonal antibody enzyme-linked immunosorbent assay Some of them were induced by PWM in vitro, BCGF_8, BCDF_3, IL-6 activity and proliferation of T and B cells were detected in the serum of INS subclasses, PnPs-specific IgG subclass antibodies, PWM-induced in vitro IgG Subclasses were significantly reduced, similar types of defects, defect frequency along the IgCH gene 5’-3 ’gradually increased trend, IgG_3 least affected, and IgG_1,