论文部分内容阅读
建立了化妆品种中2种限用呫吨染料酸性黄73和溶剂橙16的高效液相色谱/二极管阵列检测器检测方法。在ODS C18反相色谱柱上,流动相为0.1 mol/L乙酸铵-甲醇-乙腈(体积比为65:17.5:17.5)等度洗脱,检测波长为492 nm,柱温35℃,流速1.0 mL/min。以保留时间定性,外标法定量。在5～500μg/mL范围内,酸性黄73和溶剂橙16的质量浓度与各自相应的峰面积呈现良好的线性关系,仪器检出限均为0.5μg/mL。样品前处理方法采用甲醇作溶剂超声提取,酸性黄73和溶剂橙16的加标回收率分别在91.2%～106.2%和93.2%～101.5%范围内。
A method for the determination of 2 kinds of xanthene dyes Acid Yellow 73 and Solvent Orange 16 in cosmetics was established. The mobile phase was eluted with 0.1 mol / L ammonium acetate-methanol-acetonitrile (65: 17.5: 17.5 by volume) on an ODS C18 reversed-phase column with a detection wavelength of 492 nm and a column temperature of 35 ° C at a flow rate of 1.0 mL / min. To retain the time qualitative, external standard method. In the range of 5 ~ 500μg / mL, the concentrations of Acid Yellow 73 and Solvent Orange 16 showed a good linear relationship with their respective peak areas, and the detection limits were both 0.5μg / mL. The sample pretreatment method using methanol as the solvent ultrasonic extraction, acid yellow 73 and solvent orange 16 spiked recoveries were 91.2% ~ 106.2% and 93.2% ~ 101.5% range.