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目的：为了观察土贝母对Ｈｅｐ－２细胞株诱导调亡的机制。方法：应用ＭＴＴ比色法和流式细 胞仪测定细胞周期的变化，并应用电子显微镜技术观察诱导分化后亚细胞结构。结果：土贝母制剂对 Ｈｅｐ－２细胞株有明显的抑制作用，且呈剂量依赖性，流式细胞仪分析，不同的药物浓度和不同的作用时 间凋亡的发生差异均有显著意义，Ｇ１期细胞堆积，Ｓ期细胞减少，Ｇ２／Ｍ期细胞相对增多，亚细胞结构， 细胞线粒体肿胀变性，核固缩。结论：土贝母制剂能阻上Ｇ０／Ｇ１期细胞向Ｓ期转化进程，抑制Ｈｅｐ－２增 殖，促进Ｈｅｐ－２细胞凋亡。
OBJECTIVE: To observe the mechanism of induction of apoptosis in Hep-2 cell line by Fritillaria. METHODS: Changes in cell cycle were measured using MTT colorimetric assay and flow cytometry, and electron microscopy was used to observe subcellular structures after induction of differentiation. RESULTS: The Fritillaria pallidum preparation had obvious inhibitory effect on the Hep-2 cell line in a dose-dependent manner. Flow cytometry analysis showed that there were significant differences in the apoptosis of Hep-2 cells at different concentrations and different action times. G1 The cells accumulated during the period, the cells in the S phase decreased, the cells in the G2/M phase increased, the subcellular structure, the mitochondrial swelling and degeneration, and the nuclear condensation. Conclusion: Fritillaria can inhibit the transformation of G0/G1 phase cells to S phase, inhibit the proliferation of Hep-2 and promote the apoptosis of Hep-2 cells.