论文部分内容阅读
目的:建立热痹清颗粒的质量标准。方法:采用薄层色谱法(TLC)对热痹清颗粒中知母、赤芍、忍冬藤、车前草进行定性鉴别;采用反向高效液相色谱法对热痹清颗粒中马钱苷、芍药苷进行含量测定,色谱柱为Wondasil C_(18)(4.6mm×250mm,5μm),流动相为乙腈-0.2%磷酸水溶液梯度洗脱,流速为1.0mL·min~(-1),检测波长为236nm(马钱苷)、230nm(芍药苷),柱温为30℃。结果:TLC斑点清晰,分离度良好,阴性对照无干扰;马钱苷、芍药苷分别在各自进样量范围内与峰面积呈良好线性关系,相关系数均在0.999 9以上,平均加样回收率分别为100.04%、98.35%,RSD分别为1.46、1.64(n=6)。结论:该方法快速、准确、专属性强,可作为热痹清颗粒质量控制的方法。
Objective: To establish the quality standard of Bibiqing granules. Methods: The contents of loganin, Radix Paeoniae Rubra, Houttuynia cordata Thunb and the plantain were identified qualitatively by thin layer chromatography (TLC). The reverse phase high performance liquid chromatography (18) 4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile-0.2% phosphoric acid aqueous solution with a gradient of 1.0 mL · min -1 .The detection wavelength 236 nm (loganin), 230 nm (paeoniflorin), and the column temperature was 30 ° C. Results: The TLC spots were clear and the resolution was good. There was no interference with the negative control. The loganin and paeoniflorin had good linearity with the peak area in the respective injection volume, the correlation coefficients were above 0.999 9, the average recovery rate Respectively, 100.04%, 98.35%, RSD respectively 1.46,1.64 (n = 6). Conclusion: The method is rapid, accurate and specific, and can be used as a method for controlling the quality of the pellets.