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目的探讨香烟烟雾对大鼠睾丸组织一氧化氮(NO)含量、Na~+-K~+-ATP酶及Ca~(2+)-ATP酶活性的影响。方法清洁级健康雄性SD大鼠40只,随机分为对照组和低、中、高剂量染毒组(即0、10、20和30支香烟组),每组10只。染毒组大鼠置于自制被动染毒箱中,采用静式染毒方法,1次/d,连续8周。观察大鼠左侧睾丸组织形态结构,测定右侧睾丸组织匀浆中NO含量及Na~+-K~+-ATP酶、Ca~(2+)-ATP酶活性。结果各组大鼠体重增量比较,高剂量染毒组较对照组明显降低,差异有统计学意义(P<0.01);与对照组相比,高剂量染毒组大鼠睾丸中NO含量明显升高,Na~+-K~+-ATP酶和Ca~(2+)-ATP酶的活力均明显降低,差异有统计学意义(P<0.01);睾丸组织病理学发现,随着染毒剂量的增加,生精小管出现不同程度的退行性改变。结论香烟烟雾可通过改变大鼠睾丸组织中NO含量,降低Na~+-K~+-ATP酶和Ca~(2+)-ATP酶的活力,引起大鼠睾丸损伤。
Objective To investigate the effects of cigarette smoke on the content of NO, Na ~ + -K ~ + -ATPase and Ca ~ (2 +) - ATPase in testis of rats. Methods Forty healthy male Sprague Dawley rats were randomly divided into control group and low, medium and high dose groups (ie 0, 10, 20 and 30 cigarettes), 10 in each group. The rats in the exposure group were placed in a self-made passive dosing box and were treated with static exposure method once a day for 8 weeks. The morphology and structure of the left testis were observed and the content of NO and the activities of Na ~ + -K ~ + -ATPase and Ca ~ (2 +) - ATPase in the right testis homogenate were measured. Results Compared with the control group, the weight gain of rats in each group was significantly lower than that of the control group (P <0.01). Compared with the control group, the content of NO in the testis of the rats in the high dose group was significantly (P <0.01). The histopathology of testis showed that with the increase of the activity of Na ~ + -K ~ + -ATPase and Ca ~ (2 +) - ATPase, Increased doses of seminiferous tubules showed varying degrees of degenerative changes. Conclusion Cigarette smoke can reduce the content of NO in rat testis, decrease the activity of Na ~ + -K ~ + -ATPase and Ca ~ (2 +) - ATPase, and cause the testicular injury in rats.