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在室内测定了2种转 Cry1Ab 基因的Bt玉米MON810和Bt11不同组织对甜菜夜蛾 Spodoptera exigua (Hübner) 初孵幼虫以及心叶对4龄幼虫存活和发育的影响, 在田间比较了甜菜夜蛾幼虫取食 Bt 和非 Bt玉米雌穗的存活和为害情况。结果表明, 转 Cry1Ab 基因的Bt玉米的不同组织对甜菜夜蛾初孵幼虫都具有明显的杀虫活性, 取食 Bt玉米心叶、苞叶、籽粒时甜菜夜蛾均在幼虫期死亡; 取食MON810和Bt11雄穗的初孵幼虫化蛹率分别为5.2%和2.1%, 羽化率为2.1%和1.0%; 取食MON810和Bt11花丝的初孵幼虫化蛹率分别为1.0%和2.1%, 但不能羽化。4龄幼虫取食MON810玉米心叶的化蛹率与对照差异不显著, 而取食Bt11的化蛹率与对照差异显著; 取食两种Bt玉米心叶的4龄幼虫化蛹后的雌、雄蛹重和羽化率与对照组差异显著, 但蛹期和平均单雌产卵量差异不显著, 虽然对照组羽化的成虫平均产卵量高于Bt玉米组。田间接种初孵幼虫10 天后的调查结果表明, 在MON810 和 Bt11 玉米花丝上幼虫存活率分别为 1.3%和 0.3%, 而对照组分别为 12.9%和16.2%; MON810和Bt11玉米雌穗被害率分别为18.3%和5.0%, 而对照组分别为93.3%和95.0%, 均显著低于对照组。
The effects of different Cry1Ab transgenic Bt corn MON810 and Bt11 tissues on the survival and development of the 4th instar larvae of Spodoptera exigua (Hübner) were studied in the laboratory. The beet armyworm larvae Survival and Victims of Feeding Bt and Non-Bt Corn. The results showed that different tissues of transgenic Bt corn with Cry1Ab gene had obvious insecticidal activities on the larvae of the beet armyworm. The beet armyworm fed on Bt corn heart, The pupal rates of newly hatched larvae of MON810 and Bt11 were 5.2% and 2.1%, respectively, and the emergence rates were 2.1% and 1.0%, respectively. The pupal rates of newly hatched larvae fed MON810 and Bt11 were 1.0% and 2.1%, respectively, But can not be feathered. The pupation rate of the 4th instar larvae fed MON810 maize leaf was not significantly different from that of the control, but the pupation rate of the fed Bt11 was significantly different from that of the control. The pupal weight and emergence rate were significantly different from those of the control group, but there was no significant difference in pupal stage and average single female egg production, although the fecundity of females in control group was higher than that of Bt maize group. The results of 10 days after inoculation of newly hatched larvae in the field showed that the survival rates of larvae on MON810 and Bt11 corn silk were 1.3% and 0.3%, respectively, while those in the control group were 12.9% and 16.2% respectively; the earworn rates of MON810 and Bt11 maize were respectively 18.3% and 5.0%, while the control group were 93.3% and 95.0%, respectively, which were significantly lower than the control group.