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目的:研究增殖细胞核抗原(PCNA)的表达与人乳腺癌细胞凋亡的关系。方法:以乳腺癌细胞株MCF7/S(化疗敏感细胞株)为研究对象,应用MTT比色法检测阿霉素(ADR)对体外培养的MCF7/S细胞增殖抑制作用,末端标记(TUNEL)法检测ADR诱导乳腺癌细胞凋亡,免疫细胞化学法检测ADR作用前后PCNA的表达。结果:ADR抑制MCF7/S细胞增殖,呈剂量依赖性,IC50为0.128mg/L;ADR作用组MCF7/S细胞的凋亡率(Apoptoticrate,AR)为0.261,与对照组细胞的凋亡率0.0449相比明显增高(P<0.01);PCNA阳性表达率为0.3371,与对照组PCNA阳性表达率0.5152相比明显降低(P<0.01)。结论:乳腺癌肿瘤细胞的凋亡率(AR)和PCNA的阳性表达呈负相关;ADR能诱导MCF7/S细胞凋亡,并能抑制其增殖。
Objective: To study the relationship between the expression of proliferating cell nuclear antigen (PCNA) and human breast cancer cell apoptosis. Methods: The breast cancer cell line MCF7 / S (chemosensitive cell line) was used as the research object. MTT assay was used to detect the proliferation of MCF7 / S cells induced by Adriamycin (ADR). The TUNEL assay The apoptosis of breast cancer cells was detected by ADR. The expression of PCNA was detected by immunocytochemistry before and after ADR. RESULTS: ADR inhibited the proliferation of MCF7 / S cells in a dose-dependent manner with an IC50 of 0.128 mg / L. Apoptotic rate (AR) of MCF7 / S cells was 0.261 in ADR group and 0.0449 (P <0.01). The positive rate of PCNA was 0.3371, which was significantly lower than that of control group (P <0.01). Conclusion: The apoptosis rate of breast cancer cells (AR) is negatively correlated with the expression of PCNA. ADR can induce the apoptosis of MCF7 / S cells and inhibit its proliferation.