论文部分内容阅读
目的探讨P16INK4a蛋白在结直肠癌中表达情况及临床意义。方法结直肠癌患者64例,手术切除组织标本行免疫组织化学检查,观察P16INK4a蛋白表达情况,分析P16INK4a蛋白表达与临床病理特征间的关系。结果P16INK4a蛋白阴性表达12例(18.75%)、弱阳性表达4例(6.25%)、阳性表达25例(39.06%)、强阳性表达23例(35.94%),48例表达上调(75.00%);P16INK4a蛋白表达上调比例在男性(70.00%)与女性(79.41%)、高分化癌(71.74%)与低分化癌(83.33%)、临床分期≤Ⅰ期(84.62%)与临床分期≥Ⅱ期(72.55%)上差异无统计学意义(P>0.05),在无淋巴结或远处转移(88.89%)与有淋巴结或远处转移(57.14%)上差异有统计学意义(P<0.05)。结论P16INK4a蛋白表达上调是结直肠癌的重要免疫表型特征,且无淋巴结或远处转移者表达高于有淋巴结或远处转移者,可作为结直肠癌诊断及转移能力评估的重要分子靶标。
Objective To investigate the expression of P16INK4a in colorectal cancer and its clinical significance. Methods Sixty-four patients with colorectal cancer were examined by immunohistochemistry. The expression of P16INK4a protein was observed. The relationship between the expression of P16INK4a protein and clinicopathological features was analyzed. Results The positive expression rate of P16INK4a protein was 12 cases (18.75%), the weak positive expression was 4 cases (6.25%), the positive expression was 25 cases (39.06%), the strong positive expression was 23 cases (35.94%), the positive expression was 48.00% P16INK4a protein expression in the proportion of men (70.00%) and female (79.41%), well-differentiated cancer (71.74%) and poorly differentiated cancer (83.33%), clinical stage Ⅰ (84.62%) and clinical stage Ⅱ 72.55%) (P> 0.05). There was significant difference between lymph node or distant metastasis (88.89%) and lymph node or distant metastasis (57.14%) (P <0.05). Conclusion Upregulation of P16INK4a protein is an important immunophenotypic characteristic of colorectal cancer. The expression of P16INK4a protein is higher than that of lymph node or distant metastasis in patients without lymph node or distant metastasis. It may be used as an important molecular target for the diagnosis and metastasis of colorectal cancer.