论文部分内容阅读
试验比较了来自东北、华北等地小麦根腐病菌共30个菌株,从中已筛选出毒力强而稳定的菌株(002号)。初步明确采用改良Fries加小麦健叶汁的液体培养基培养根腐茵,每天振荡4次,每次20min,培养15d后的培养物滤液,在5℃下可保存2个月,用15磅高压灭菌30min及煮沸浓缩均不影响毒素的作用。在几种Hs—毒素制剂中,以粗提物制剂的毒素作用最强,病叶提取液和培养滤液次之。应用根冠细胞测定法评价Hs—毒素的作用比用抑制种子根伸长的种苗“萌发包”法更为灵敏,精确,快速。测定小麦离体:浪冠细胞的最佳条件是:黑暗25℃下处理6h;根冠细胞浓度为500—1000个/ml;粗提毒素稀释液pH6—7。
A total of 30 strains of Triticum aestivum were collected from northeast China and North China. The strains with strong virulence and stability (002) were screened out. Root-rot fungus was cultured in a liquid medium with modified Fries plus wheat leaf juice, shaken 4 times a day for 20 minutes, and the culture filtrate after 15 days of culture was stored at 5 ° C. for 2 months. Sterilization 30min and boiling concentrated does not affect the role of toxins. Among several Hs-toxin preparations, the toxin was the strongest in the crude extract preparation, followed by the diseased leaf extract and the culture filtrate. The application of root-cap cell assay to evaluate the effect of Hs-toxin was more sensitive, accurate and rapid than that of seedling “germination package” that inhibited seed-root elongation. The optimal conditions for the determination of wheat in vitro were as follows: the darkness was treated at 25 ° C for 6 hours; the concentration of root and crown cells was 500-1000 cells / ml; the crude toxin diluted solution was pH6-7.