PCR扫描筛选技术以保守序列为基础设计引物,以基因文库为模板进行扩增,获得新型活性微生物代谢产物的基因,继而通过基因重组技术获得对应活性代谢产物,克服了传统筛选方法必须获得微生物纯培养物的限制,有效提高新型活性微生物代谢产物发现的概率。本文综述利用PCR扫描技术筛选新型活性微生物代谢产物的基本方法及其在聚酮类化合物和新型酶类筛选中的应用进展。 PCR scans screening technology based on the conservative sequence design primers, gene library as a template for amplification, access to new active metabolites of microbial genes, and then obtained by gene recombinant technology to the corresponding active metabolites, to overcome the traditional screening methods must be pure microbial Culture restrictions, effectively increase the probability of new active microbial metabolites found. This review summarizes the basic methods for the screening of novel metabolites of active microorganisms by PCR and their applications in the screening of polyketides and novel enzymes.