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目的 检测燃煤性砷中毒患者外周血红细胞GPA的突变频率及其与病程的关系。方法 分离、固定4 0例燃煤性砷中毒患者外周血红细胞 ,与荧光素标记的单抗结合后 ,采用流式细胞术进行GPA变异分析 ,计算GPA变异频率。结果 4 0例燃煤性砷中毒患者外周血GPANN变异频率为 (2 1.2 3± 13.97)× 10 -6,NO变异频率为 (33.13± 2 5 .72 )× 10 -6,MM变异频率为 (110 .90± 6 3.5 8)× 10 -6,MO变异频率为 (2 0 .35± 2 1.2 6 )× 10 -6,GPA变异频率明显高于正常人群 (P <0 .0 1) ,但不同病程的燃煤性砷中毒患者之间的GPA变异频率未见明显差异。结论 砷中毒可导致人类外周血红细胞GPA的突变。提示GPA的突变频率可作为一项检测有否砷中毒的指标
Objective To detect the frequency of GPA mutation in peripheral blood red blood cells and its relationship with the course of disease in patients with arsenic poisoning. Methods Peripheral blood erythrocytes were isolated and fixed in 40 patients with arsenic poisoning. After being combined with fluorescein - labeled monoclonal antibody, GPA mutation was analyzed by flow cytometry, and the frequency of GPA mutation was calculated. Results The frequency of GPANN mutation in peripheral blood was (2 1.2 3 ± 13.97) × 10 -6 and the frequency of NO mutation was (33.13 ± 2.57.2) × 10 -6 in 40 patients with arsenic poisoning. The variation frequency of MM was ( 110 .90 ± 6 3.58) × 10 -6. The mutation frequency of MO was (20.35 ± 2 1.2 6) × 10 -6, and the frequency of GPA mutation was significantly higher than that of the normal population (P <0.01) There was no significant difference in the frequency of GPA mutations between patients with different stages of arsenic poisoning. Conclusion Arsenism can result in the mutation of GPA in human peripheral erythrocytes. Tip GPA mutation frequency can be used as a measure of whether there is arsenic poisoning indicators