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探讨Th2相关细胞因子IL 4、IL 1 0与血小板相关抗体PAIgG、PAIgM在儿童ITP的发病机理中的作用。方法是采用双抗体夹心酶联免疫吸附试验 [ELISA]技术检测 30例ITP患儿血浆中IL 4、IL 1 0水平 ,同时检测PAIgG、PAIgM的水平 ,研究IL 4、IL 1 0对PAIgG、PAIgM的调节作用。结果显示 :( 1 )急性期ITP患儿IL 4、PAIgG、PAIgM水平均高于对照组 (P <0 .0 5)。( 2 )慢性ITP病例半年后IL 4、PAIgG、PAIgM仍然高于对照组 (P <0 .0 5) ,IL 4与血小板相关抗体水平呈显著正相关。 ( 3)病程中IL 1 0与对照组无明显变化。结论 :ITP患儿IL 4表达增加 ,提示Th2相关细胞因子比例失调 ,使血小板相关抗体增高为重要的致病因素之一。
To investigate the role of Th2-related cytokines IL-4, IL-10 and platelet-associated antibodies PAIgG and PAIgM in the pathogenesis of childhood ITP. IL-10 and IL-10 levels in plasma were measured by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) in 30 children with ITP. Levels of PAIgG and PAIgM were measured at the same time. The effects of IL 4 and IL 10 on PAIgG, PAIgM The regulatory role. The results showed that: (1) The levels of IL 4, PAIgG and PAIgM in children with acute ITP were higher than those in the control group (P <0.05). (2) The levels of IL 4, PAIgG and PAIgM in chronic ITP patients were still higher than those in control group (P <0.05), IL 4 and platelet-related antibody levels were positively correlated. (3) There was no significant difference between IL 10 and control group in the course of disease. CONCLUSION: The increased expression of IL-4 in children with ITP suggests that the imbalance of Th2-related cytokines and the increase of platelet-associated antibodies are one of the important risk factors.