论文部分内容阅读
目的观察异丙酚对大鼠不完全脑缺血再灌注后脑钙代谢和脑水肿程度的影响。方法SD大鼠40只平均分成5组,对照组、缺血组、缺血再灌注组、给异丙酚后缺血组、给异丙酚后缺血再灌注组。以颈动脉夹闭伴低血压(6.67kPa)制作不完全性脑缺血,以放开颈动脉夹并使血压恢复至缺血前水平为再灌注。取脑皮质测定脑水和钙含量。结果脑缺血后脑皮质水和钙含量升高(P<0.05),再灌注60min后,两指标进一步升高(P<0.01)。异丙酚能明显降低缺血和再灌注后脑钙和水含量升高程度(P<0.05),对脑含水量影响与其对脑钙影响呈正相关(r=0.917,P<0.01)。结论异丙酚能降低不完全脑缺血再灌注后脑皮质钙含量和脑水肿程度,对不完全性脑缺血有一定保护作用,其机制可能与降低脑缺血后脑细胞钙超载有关
Objective To observe the effect of propofol on cerebral calcium metabolism and cerebral edema after incomplete cerebral ischemia-reperfusion in rats. Methods 40 SD rats were equally divided into 5 groups: control group, ischemia group, ischemia-reperfusion group, propofol-induced ischemia group and propofol-ischemia-reperfusion group. Incomplete cerebral ischemia was made with carotid artery occlusion and hypotension (6.67 kPa) to release the carotid artery clip and return the blood pressure to the pre-ischemic level for reperfusion. Cortical brain and calcium content determination. Results Cerebral cortex water and calcium levels increased after cerebral ischemia (P <0.05). After 60 min of reperfusion, both indexes increased further (P <0.01). Propofol significantly decreased the level of brain calcium and water after ischemia and reperfusion (P <0.05), and had a positive correlation with brain water content (r = 0.917, P <0. 01). Conclusion Propofol can reduce cerebral cortex calcium content and cerebral edema after incomplete cerebral ischemia and reperfusion, which may have a protective effect on incomplete cerebral ischemia. Its mechanism may be related to reducing calcium overload of brain cells after cerebral ischemia