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水稻(Oryza sativa)是我国重要的粮食作物之一。水稻矮秆材料的引入掀起了第1次“绿色革命”。但近年来,在水稻育种中矮生基因遗传单一的问题越来越突出,已经严重影响到水稻产量的持续提高。利用60Co-γ射线辐照籼稻亲本材料M804获得了一个性状能够稳定遗传的矮秆突变体MU101。对该矮秆突变体和台粳16号杂交获得的F2代的遗传分析表明,该矮秆性状受1对隐性单基因控制,并暂命名为ds1。利用已有的SSR分子标记将DS1基因定位在水稻第5号染色体上,通过扩大群体和开发新的Indel标记,进一步将DS1基因定位在2个Indel标记之间,两者间的物理距离大约为384kb。该研究为DS1基因的克隆及其在生产中的应用奠定了基础。
Oryza sativa is one of the important food crops in our country. The introduction of rice dwarf material set off the first “green revolution”. However, in recent years, the problem of single inheritance of dwarf gene has become more and more prominent in rice breeding, which has seriously affected the continuous increase of rice yield. A dwarf mutant MU101 with stable trait was obtained by irradiating 60Co-γ ray on indica rice M804. The genetic analysis of the F2 generation obtained by crossing the dwarf mutant with Tajing 16 showed that the dwarf trait was controlled by a single recessive single gene and was tentatively named ds1. Using the existing SSR molecular markers to locate the DS1 gene on rice chromosome 5, by expanding the population and developing a new Indel marker, further locating the DS1 gene between the two Indel markers, the physical distance between them is approximately 384kb. This study laid the foundation for the cloning of DS1 gene and its application in production.