论文部分内容阅读
目的:对分离自海南红树林真菌菌株PH0016的胞外多糖(exopolysaccharide,EPS)抗单纯疱疹病毒Ⅱ型(Herpes simplex virus type 2,HSV-2)活性进行研究。方法:收集纯化菌株PH0016产生的EPS,体外采用细胞病变观察EPS的细胞毒性和抗HSV-2作用。为观察EPS对HSV-2体内感染的治疗效果,小鼠颅内注射0.02ml滴度为100LD50/0.1ml-1的HSV-2,24小时后以不同浓度EPS灌胃,持续7天,14天后计算小鼠存活率和存活时间。菌株分类采用形态学观察和ITS序列分析。结果:EPS可抑制HSV-2病毒活性,病毒感染的细胞病变的半抑制浓度(Inhibited concentration,IC50)为313μg/mL,SI为11.43。EPS 25mg.kg-1.d-1灌胃后,小鼠存活率为40.0%,存活时间为9.82±1.87天,相比模型组实验动物存活时间和存活率均有提高。菌株初步鉴定为淡紫色拟青霉。结论:从海南红树林分离一株淡紫色拟青霉,其产生的EPS具有抗HSV-2活性,值得进一步研究。
Objective: To study the anti-herpes simplex virus type 2 (HSV-2) activity of exopolysaccharide (EPS) isolated from Hainan mangrove fungus strain PH0016. Methods: The EPS produced by the purified strain PH0016 was collected and the cytotoxicity of EPS and the anti-HSV-2 effect were observed by cytopathic effect in vitro. To observe the therapeutic effect of EPS on HSV-2infection in vivo, mice were intraperitoneally injected with 0.02ml of HSV-2 with a titer of100LD50 / 0.1ml-1 for24h and then gavaged with different concentrations of EPS for7days and14days Mouse survival rate and survival time were calculated. Strains were classified by morphological observation and ITS sequence analysis. Results: EPS inhibited the activity of HSV-2 virus. The IC50 of cytopathic effect was 313μg / mL and SI was 11.43. After gavage, the survival rate of mice was 40.0% and the survival time was 9.82 ± 1.87 days. Compared with the model group, the survival time and survival rate of experimental animals increased. The strain was initially identified as Paecilomyces lilacinus. Conclusion: Isolation of a Paecilomyces lilacinus from Hainan mangrove, its EPS production has anti-HSV-2 activity, worth further study.