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目的 观察海人藻酸 (KA)对大鼠基底前脑含乙酰胆碱能神经元 (包括其投射纤维投射部位 )毁损后的数目和形态改变 ,以探讨何首乌 (PMT)对胆碱能神经元及其纤维的保护作用及机制。方法 采用兴奋性神经毒素海人藻酸 (KA)损伤基底前脑 (BF)Meynert核团 ,内侧隔核 (MS)及斜角带核 (DB) ,建立毁损模型 ,用何首乌喂饲毁损实验组 ,使用组织化学方法显示乙酰胆碱能神经元及纤维的改变及可能的用药后保护和活化作用。结果 何首乌喂饲组 (A组 )比非何首乌喂饲组 (B组 )在基底前脑中的Meynert核数量多 ,且细胞完整无破坏 ,其投射到海马及大脑皮质的AChE纤维数目多 ,纤维形态无破坏 ,差异有显著性 (P <0 .0 1)。结论 何首乌对大鼠AChE神经元及其投射纤维有保护作用。
Objective To observe the number and morphological changes of acetylcholineergic neurons (including their projected fiber projection sites) in rat basal forebrain after kainic acid (KA) in order to investigate the effect of Polygonum multiflorum (PMT) on cholinergic neurons. Fiber protection and mechanism. Methods The excitatory neurotoxin kainic acid (KA) was used to damage the basal forebrain (BF) Meynert nuclei, the medial septum (MS) and the oblique nucleus (DB), and the damage model was established. The experimental group was fed with Polygonum multiflorum. The use of histochemical methods revealed changes in acetylcholineergic neurons and fibers and possible protection and activation after administration. Results The number of Meynert nucleus in the basal forebrain was higher than that of the non-polygonum multiflorum fed group (group A) than that of the non-polygonum multiflorum fed group (group B), and the cell integrity was intact. The number of AChE fibers projected to the hippocampus and cerebral cortex was much, and the fibers The morphology was non-destructive and the difference was significant (P < 0.01). Conclusion Polygonum multiflorum has a protective effect on rat AChE neurons and their projection fibers.