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目的建立评价荜澄茄质量的GC指纹图谱分析方法。方法采用水蒸气蒸馏法提取挥发油,GC测定14批荜澄茄药材。气相色谱条件:DB-Wax石英毛细管色谱柱(30 m×0.25 mm,0.25μm),氢火焰离子化检测器(FID),进样口温度250℃,检测器温度250℃,柱温起始温度为50℃,以3℃·min-1升温至75℃,以10℃·min-1升温至130℃,以5℃·min-1升温至240℃,维持15 min。载气为氮气,流速为1.0 mL·min-1,进样方式为分流进样,分流比10∶1,进样量为1μL。结果依据14批药材的指纹图谱数据进行聚类分析,将样品分为2类,选定其中的12批优质样品建立共有模式,相似度分析结果表明,第Ⅰ类为优质药材,第Ⅱ类为一般品。结论本法专属性强、重复性好,为科学评价与鉴定荜澄茄药材质量提供了依据。
Objective To establish a method of GC fingerprinting to evaluate the quality of Ctenopharyngodon idellus. Methods Volatile oil was extracted by steam distillation. 14 batches of 荜 茄 茄 herbs were determined by GC. Gas chromatographic conditions: DB-Wax quartz capillary column (30 m × 0.25 mm, 0.25 μm), flame ionization detector (FID), inlet temperature of 250 ° C, detector temperature of 250 ° C, The temperature was raised to 75 ° C at 3 ° C · min-1, the temperature was raised to 130 ° C at 10 ° C-min-1, and the temperature was raised to 240 ° C at 5 ° C-min-1 for 15 minutes. The carrier gas is nitrogen and the flow rate is 1.0 mL · min-1. The injection mode is split injection, the split ratio is 10: 1, and the injection volume is 1 μL. Results Based on the fingerprints of 14 batches of medicinal materials, the samples were divided into two groups. Twelve batches of high quality samples were selected to establish the common mode. The similarity analysis showed that the first category was high quality medicinal materials, the second category was General goods. Conclusion This method is highly specific and reproducible, which provides a scientific basis for the scientific evaluation and identification of the quality of 荜 茄 茄 medicinal materials.