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建立用于测定金丝桃苷血药浓度的超高效液相-质谱联用分析方法(UPLC-MS),应用超高效液相-质谱法,采用电喷雾离子源(ESI),负离子模式检测,对大鼠血浆中的金丝桃苷进行定量测定,并研究金丝桃苷静脉给药后在大鼠体内的药代动力学过程。在10分钟内完成大鼠血浆样品内金丝桃苷的分析。金丝桃苷线性关系良好(r2>0.999),日间差和日内差小于15%,定量限为4 ng/mL,符合生物样品分析要求。本方法的定量限低于文献报道,且所需的血浆体积只有50μL,优于文献方法。本方法可成功应用于大鼠静脉给药后金丝桃苷的血药浓度测定,为金丝桃苷临床应用及临床药物监测提供帮助。
To establish a UPLC-MS method for the determination of the concentration of hyperoside in plasma, the effects of hyperoside HPLC-MS, ESI, negative ion mode, The quantitative determination of hyperin in rat plasma and the pharmacokinetics of hyperin in rats after intravenous administration were studied. The analysis of the hyperin in rat plasma samples was completed within 10 minutes. Hyperine showed a good linearity (r2> 0.999) with a difference of less than 15% in daytime and daytime, with a limit of quantification of 4 ng / mL, which met the requirements of biological sample analysis. The limit of quantitation of this method is lower than that reported in the literature, and the required plasma volume is only 50 μL, which is better than the literature method. The method can be successfully applied to the determination of hyperoside in rats after intravenous administration, which can be used for the clinical application of hyperin and monitoring of clinical drugs.