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目的:对灯盏花群体种质资源进行分析,为灯盏花的品种选育提供依据。方法:用筛选出来的11对引物对采自云南泸西、昆明、大理、丘北、石林的6份灯盏花种质资源进行AFLP遗传多样性分析。结果:①11个引物在6份种质资源中共产生636条DNA片段,其中604条带为多态性条带,约占82.40%。②在相似系数为0.706时,6份不同地区的灯盏花可聚为3类,第1类包括千山1号和千山2号大部分样品及大理、石林、昆明3个地区的野生居群;第2类为丘北的野生居群;第3类主要为部分千山2号及其他地区样品。千山1号和千山2号灯盏花平均遗传相似系数大,遗传相似系数变幅小,品系内遗传分化较小,遗传性状较稳定;③对6份灯盏花样品的分子系统聚类分析表明:地理来源相同的部分有相对聚合现象或少部分品系出现交差聚类,与其亲缘关系较近有关;丘北居群自行一类,与其特异的遗传基础差异较大有关。结论:AFLP对灯盏花的遗传多样性分析具有可靠性;系统选育对灯盏花的育种具有可行性。
OBJECTIVE: To analyze the germplasm resources of Erigeron breviscapus and provide the basis for Breeding of Breviscapus species. Methods: AFLP genetic diversity of 6 elder flowers collected from Luxi, Kunming, Dali, Qiu Bei and Shilin in Yunnan Province was analyzed with 11 pairs of primers. Results: ①11 primers generated 636 DNA fragments in 6 germplasm resources, of which 604 bands were polymorphic, accounting for 82.40%. ② When the similarity coefficient was 0.706, Breviscapus in 6 different regions could be grouped into three categories. The first category included most of the samples of Qianshan 1 and Qianshan 2 and the wild populations of Dali, Shilin and Kunming ; The second category is the wild population of Qiu Bei; the third category is mainly Qianshan 2 and other regional samples. Qianshan 1 and Qianshan 2 were the largest genetic similarity coefficient, genetic similarity coefficient amplitude small, less genetic differentiation within the strain, genetic traits more stable; ③ 6 Fenreng sample molecular phylogenetic analysis showed : The same geographical origin of the relative aggregation phenomenon or a small number of cross-clustering lines appear, and their close relatives related; Qiu Bei population on their own class, and its specific genetic basis for the greater differences. Conclusion: The genetic diversity of Erigeron Breviscapus AFLP is reliable. Breeding of Erigeron breviscapus by systematic breeding is feasible.