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目的 探讨乙肝患者肝组织中乙型肝炎病毒（HBV ） cccDNA与血清 HBV DNA及乙肝病毒e抗原（HBeAg）含量的关系。方法 采用实时荧光定量聚合酶链反应检测乙肝患者肝组织 HBV cccDNA和血清HBV DNA ，采用化学发光法检测其血清中 HBeAg。结果 肝组织HBV cccDNA与血清HBV DNA定量呈正相关（ r ＝0．806，P ＜0．01），与血清 HBeAg定量值无相关关系（ r ＝0．219，P ＞0．05）。结论 血清HBeAg阳性组病毒复制较阴性组活跃，HBV DNA阴性不能完全反映肝组织内 HBV是否存在复制，而检测肝组织HBV cccDNA可精确反映肝内 HBV复制情况。“,”Objective]To explore the relationship of hepatitis B virus(HBV) cccDNA with serum HBV DNA and HBeAg in liver tissues of patients with hepatitis B .[Methods]HBV cccDNA and serum HBV DNA in liver tis‐sues of patients with hepatitis B were detected by real‐time fluorescence quantitative polymerase chain reaction ,and serum HBeAg was detected by chemiluminescence method .[Results]HBV cccDNA in liver tissue was positively cor‐related with serum HBV DNA( r=0 .806 ,P<0 .01) ,but was not correlated with serum HBeAg( r=0 .219 ,P<0 .05) .[Conclusion]Virus replication in serum HBeAg positive group is more active than that in serum HBeAg nega‐tive group .Negative HBVDNA can not fully reflect HBV replication in liver tissues ,but the detection of HBV cccD‐NA in liver tissues can accurately reflect the replication of intrahepatic HBV .