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目的:探讨精液分析区域性外部质量控制实践中参评实验室检测结果的准确性和结果的可比性。方法:制作2个水平的精子浓度、活动率和正常形态率的质控品发放给参评实验室进行外部质量控制测试,分析质控结果。评价指标为均值和实验室间变异系数(CV)。结果:精子浓度、前向运动精子百分率(PR)和正常形态精子百分率(NR)CV分别为34.09%、24.96%和62.01%;各实验室精子浓度、PR结果差异均无统计学意义(P>0.05),NR差异有统计学意义(P<0.05)。结论:外部质控结果CV较大,尤其正常形态精子的检测结果,提示技术人员亟需进行规范培训,开展标准化精液分析质量控制活动,提高分析结果的重复性和准确性。
OBJECTIVE: To investigate the accuracy and comparability of the laboratory test results obtained in the regional external quality control practice of semen analysis. Methods: Two levels of sperm concentration, activity and normal morphology of quality control were distributed to the laboratory for external quality control testing, analysis of quality control results. Evaluation indicators for the mean and inter-laboratory coefficient of variation (CV). Results: The spermatozoa concentration, the percentage of forward motile spermatozoa (PR) and the percentage of normal spermatozoa (NR) were 34.09%, 24.96% and 62.01% respectively. There was no significant difference in sperm concentration and PR between labs (P> 0.05), NR difference was statistically significant (P <0.05). Conclusion: The results of external quality control CV is large, especially the test results of normal morphology sperm, suggesting that technical personnel urgently need to conduct standardized training and carry out standardized semen analysis quality control activities to improve the repeatability and accuracy of the analysis results.