论文部分内容阅读
目的:观察不同剂量甲泼尼龙治疗大鼠百草枯中毒肾脏损伤的疗效。方法:将120只Wistar大鼠随机分为五组,空白组,染毒组和干预组(根据甲泼尼龙剂量不同分为三组),除空白组外,均予百草枯(22mg/kg)稀释后腹腔注射,2h后依照组别、体重注射甲泼尼龙,在第1、3、7天共3个时间点,按抽签法处死实验对象6只获取标本,观察肾功能和病理变化。结果:各组血尿素氮(P=0.001<0.05)和肌酐(P=0.01<0.05)差异有统计学意义,干预组中5mg/kg甲泼尼龙组同染毒组比较差异有统计学意义。不同时间点血尿素氮(P=0.007<0.05)和肌酐(P=0.016<0.05)差异有统计学意义,其中第七天明显低于第一、三天。病理评分各组(P=0.21>0.05)差异无统计学意义。讨论:早期应用糖皮质激素治疗PQ中毒大鼠,可以显著减轻PQ中毒所致的肾损伤程度,改善肾功能,尤其小剂量改善显著,传统的大剂量糖皮质激素冲击治疗不值的推崇。
Objective: To observe the curative effect of different doses of methylprednisolone on renal damage of paraquat poisoning in rats. Methods: One hundred and twenty Wistar rats were randomly divided into five groups: blank group, exposure group and intervention group (divided into three groups according to methylprednisolone dosage). Paraquat (22 mg / kg) After 2h, methylprednisolone was injected according to the group and body weight. After 3, 7 days and 3 days, 3 rats were killed by the method of drawing lots to get the specimens, and the renal function and pathological changes were observed. Results: The differences of blood urea nitrogen (P = 0.001 <0.05) and creatinine (P = 0.01 <0.05) in each group were statistically significant. The difference was statistically significant between the intervention group and the control group. The blood urea nitrogen (P = 0.007 <0.05) and creatinine (P = 0.016 <0.05) had statistical significance at different time points, of which the seventh day was obviously lower than the first and third days. Pathological score of each group (P = 0.21> 0.05) no significant difference. Discussion: The early application of glucocorticoid treatment of PQ poisoning rats, can significantly reduce the degree of renal damage caused by PQ poisoning and improve renal function, especially in small doses to improve significantly, the traditional high-dose glucocorticoid impact therapy is not worth respected.