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MST1是死亡受体信号通路Hippo通路的核心成员,其基因表达异常多见于结直肠癌、肝癌、胃癌等肿瘤。为了探讨MST1表达对人结直肠癌SW480细胞增殖与凋亡的影响及其发生机制,采用PolyJet TM介导pEGFP-N1-Mst1及LipofectamineTM2000介导Mst1特异性-siRNA转染至SW480细胞,分别建立高低表达Mst1的细胞模型。对不同分组的细胞增殖、凋亡及凋亡相关蛋白的表达进行检测。结果显示,Mst1高表达组细胞增殖抑制、凋亡率及凋亡相关蛋白的表达显著增高,反之,Mst1低表达组细胞增殖加快,细胞凋亡率及凋亡相关蛋白的表达显著降低。结果提示,Mst1的靶向调控能较好地控制结直肠癌细胞的增殖与凋亡,可作为人结直肠癌防治的新研究靶点。
MST1 is a core member of the Hippo pathway of death receptor signaling. Its gene expression is more common in colorectal cancer, liver cancer and gastric cancer. In order to investigate the effect of MST1 on the proliferation and apoptosis of human colorectal cancer SW480 cells and its mechanism, PolyJet ™ -mediated pEGFP-N1-Mst1 and LipofectamineTM2000 were used to transfect Mst1-specific siRNA into SW480 cells, Mst1-expressing cell model. Different groups of cell proliferation, apoptosis and apoptosis-related protein expression were detected. The results showed that Mst1 overexpression significantly inhibited the proliferation, the apoptosis rate and the expression of apoptosis-related proteins. In contrast, Mst1 low expression group accelerated proliferation, apoptosis rate and apoptosis-related protein expression was significantly reduced. The results suggest that the targeted regulation of Mst1 can better control the proliferation and apoptosis of colorectal cancer cells, which can be used as a new research target for the prevention and treatment of colorectal cancer.