采用套式逆转录聚合酶链反应（ＲＴｎＰＣＲ）法，用５′端引物对我国部分不同地区ＨＧＶＲＮＡ阳性的血清进行扩增，扩增产物为３９１ｂｐ，将其纯化后连接到ｐＧＥＭＴＥａｓｙ质粒上，然后进行测序。测序结果表明，５′非编码区可分为两部分，靠近５′端为保守区，靠近核心区５０ｂｐ为高度变异区，而靠近５′非编码区的部分核心区的序列也较为保守。５株中国南方ＨＧＶ的５′端基因序列与美国报道的ＧＢＶＣ和ＨＧＶ的同源性分别在８３．４％～８９．１％和８５．７％～９０．３％，而５株之间的同源性超过９１．４％，显示中国的ＨＧＶ不同于美国报道的ＧＢＶＣ和ＨＧＶ，而且在中国流行的ＨＧＶ之间的基因序列较为保守。 Using reverse transcriptase polymerase chain reaction (RTnPCR) method, the 5 ’end primer was used to amplify the HGV RNA positive sera in some areas of China, and the amplified product was 391bp. After purification, it was ligated into pGEMEasy plasmid On, and then sequenced. Sequencing results showed that the 5 ’untranslated region could be divided into two parts, near the 5’ end, close to the 50 bp in the core region as a highly variable region, while some of the core regions close to the 5 ’non-coding region were also conserved. The 5 ’end sequences of HGV from 5 southern China were 83.4% -89.1% and 85.7% -90.3% homologous to the reported GBVC and HGV in the United States, respectively. However, The homology between them is more than 91.4%, indicating that HGV in China is different from the reported GBV-C and HGV in the United States, and the gene sequences between HGVs that are popular in China are relatively conservative.