论文部分内容阅读
为分析感染Cy HV-2异育银鲫(Carassius auratus gibelio)体内不同器官组织结构和功能的损伤,采集健康和患病异育银鲫头肾、脾脏、体肾、肠道、肝胰脏、鳃各组织样本,通过石蜡切片,HE染色对各器官组织进行组织病理学观察并对肠道和鳃进行扫描电镜观察。病鱼主要表现为,食欲减弱、离群独游、全身发黑、背鳍、尾鳍末端发白、眼球突出、肌肉充血,鳃呈鲜红色。在鳃丝末端由红色斑块,经解剖有红色腹水流出,组织器官广泛性损伤。组织病理学变化为,头肾和脾脏细胞溶解、免疫细胞侵润、免疫和造血系统严重破坏;体肾中肾小球萎缩、肾小管上皮细胞脱落、肾间质出现空泡、免疫细胞侵润;肠道绒毛增生、绒毛和微绒毛长度显著减少、粘膜下层显著增厚;肝胰脏中肝细胞出现溶解、中央静脉受损;鳃中毛细血管破坏、血管破裂或扩大、红细胞溢出、鳃小片基部细胞显著减少。结果表明,病毒对异育银鲫具有明显的致病性,可导致各器官组织发生病变,同时机体处于缺血、缺氧、营养不足、废物积累的内环境,这进一步加剧对异育银鲫主要的器官组织的损伤。
In order to analyze the damage of the structure and function of different organs in CyV HV-2 allogynogenetic crucian carp Carassius auratus gibelio, the head kidney, spleen, body kidney, intestine, liver and pancreas of healthy and diseased silver crucian carp were collected, Gill tissue samples, paraffin sections, HE staining of various organs and tissues histopathological observation of the gut and intestinal gland scanning electron microscopy. Diseased fish mainly manifested as weakened appetite, outing solitary tour, black body, dorsal fin, tail fin caudal, prominent eyeballs, muscle congestion, bright red gills. At the end of gill filaments by the red plaque, dissected red ascites outflow, extensive tissue and organ damage. Histopathological changes were head and kidney and spleen cell lysis, invasion of immune cells, severe damage of immunity and hematopoietic system, glomerular atrophy in renal tubule, shedding of renal tubular epithelial cells, vacuolization of renal interstitium, infiltration of immune cells ; Intestinal villi hyperplasia, villi and microvilli significantly reduced the length of the submucosa significantly thickening; hepatocytes in liver and pancreas dissolved, central venous damage; gill capillaries destruction, rupture or enlargement of blood vessels, red blood cell spill, gill pieces Basal cells were significantly reduced. The results showed that the virus had obvious pathogenicity on allogynogenetic crucian carp, which could lead to pathological changes in various organs and tissues, meanwhile the body was in the internal environment of ischemia, hypoxia, undernutrition and waste accumulation, Major organ tissue damage.