论文部分内容阅读
目的:观察大鼠肝缺血／再灌注(I／R)损伤中肝组织肿瘤坏死因子—a(TNF—a)、白介素—6(IL—6)含量的变化。方法:建立肝I／R损伤动物模型。选择健康Wistar大鼠48只,随机分为6组即对照组(CTL)、缺血30min组(I组)和4组缺血30min再灌注组(I／R、I／RIh、I／R2h、I／R4h)。应用放射免疫方法分别测定肝组织TNF—a、IL—6含量的变化。结果:肝组织TNF—a．IL—6的含量在I／R、I／RIh、I／R2h、I／R4h组分别与对照组、I组比较差异有统计学意义,I／RIh、I／R2h、I／R4h组与I／R组比较差异有统计学意义,I／R2h、I／R4h组与l／R1h组比较差异无统计学意义,I／RIh组增高明显。结论:随着肝I／R损伤时闻的延长,肝组织TNF—a、IL—6的含量逐渐增加,TNF—a、IL—6参与了肝缺血／再灌注损伤的发生机制。
Objective: To observe the changes of tumor necrosis factor-a (TNF-a) and interleukin-6 (IL-6) in rat liver ischemia / reperfusion (I / R) injury. Methods: To establish animal model of liver I / R injury. Forty-eight healthy Wistar rats were randomly divided into 6 groups (CTL group), ischemia group (group I) and ischemia reperfusion group (I / R group, I / RIh group, I / I / R4h). The levels of TNF-a and IL-6 in liver tissues were measured by radioimmunoassay. Results: Liver tissue TNF-a. The contents of IL-6 in I / RI, I / RIh, I / R2h and I / R4h groups were significantly different from that in control group and I group / R group was statistically significant difference, I / R2h, I / R4h group and l / R1h group was no significant difference, I / RIh group increased significantly. CONCLUSION: With the prolongation of liver I / R injury, the levels of TNF-a and IL-6 in liver tissue gradually increase. TNF-a and IL-6 are involved in the pathogenesis of hepatic ischemia / reperfusion injury.