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目的:观察马齿苋提取物对肺癌A549细胞增殖的影响。方法:采用MTT比色法观察马齿苋提取物抑制肺癌A549细胞增殖;采用流式细胞仪检测马齿苋对肺癌A549细胞周期分布的影响。结果:马齿苋提取物可抑制肺癌A549细胞增殖,其作用具有时间和浓度依赖性;马齿苋提取物可诱导细胞凋亡;同时改变细胞周期分布,多数细胞阻滞于G2/M期,与对照组比较,细胞凋亡率差异有显著性(P<0.01)。结论:马齿苋提取物可诱导肺癌A549细胞凋亡,改变细胞周期分布,从而抑制细胞增殖。
Objective: To observe the effect of purslane extract on the proliferation of lung cancer A549 cells. Methods: MTT assay was used to observe the effect of Portulaca oleracea extract on the proliferation of lung cancer A549 cells. Flow cytometry was used to detect the effect of Portulaca oleracea on the cell cycle distribution of A549 lung cancer cells. RESULTS: Portulaca oleracea extract could inhibit the proliferation of lung cancer A549 cells in a time- and concentration-dependent manner. Portulaca oleracea extract could induce apoptosis, while changing cell cycle distribution, and most cells arrested in G2/M phase. Compared with the control group, the apoptosis rate was significantly different (P<0.01). Conclusion: Portulaca oleracea extract can induce apoptosis of lung cancer A549 cells, change cell cycle distribution, and thus inhibit cell proliferation.