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目的研究三氯乙烯对大鼠肝细胞DNA损伤和肝组织氧化应激的作用。方法选用雄性SD大鼠,每组5只,用1500和3000 mg/kg的三氯乙烯经口灌胃染毒,48 h处死动物。用单细胞凝胶电泳(彗星实验)检测肝细胞DNA损伤,并检测肝组织中活性氧(ROS)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量。结果三氯乙烯在1500和3000 mg/kg剂量条件下,肝细胞DNA的Olive尾矩增加,DNA损伤细胞率分别为41.96%和54.13%,也明显高于对照组的9.30%(P<0.01);大鼠肝组织中的ROS、MDA均较对照组显著增高(P<0.01),同时GSH又较对照组明显下降(P<0.01)。结论三氯乙烯在1500和3000 mg/kg剂量条件下染毒大鼠后,造成肝组织明显的氧化应激并引起肝细胞DNA损伤。
Objective To study the effect of trichlorethylene on DNA damage and liver tissue oxidative stress in rat hepatocytes. Methods Male Sprague-Dawley rats were randomly divided into five groups. The animals were orally administered with trichlorethylene at doses of 1500 and 3000 mg / kg, and animals were sacrificed at 48 hours. The DNA damage of hepatocytes was detected by single cell gel electrophoresis (comet assay), and the contents of reactive oxygen species (ROS), malondialdehyde (MDA) and reduced glutathione (GSH) in liver tissue were detected. Results At the doses of 1500 and 3000 mg / kg, the Olive tail moment of hepatocyte DNA increased and the rate of DNA damage cells was 41.96% and 54.13%, respectively, which was also significantly higher than that of the control group (9.30%, P <0.01) ; The levels of ROS and MDA in the liver of rats were significantly higher than those of the control group (P <0.01), and GSH was significantly lower than that of the control group (P <0.01). Conclusion Trichlorethylene in rats exposed to 1500 and 3000 mg / kg dose resulted in significant oxidative stress in liver tissue and DNA damage in hepatocytes.