论文部分内容阅读
目的 :探讨烟草成份保护多巴胺神经元对抗 6 羟基多巴胺 (6 OHDA)的神经毒性作用。方法 :采用大鼠脑内立体注射 6 OHDA建立帕金森病模型 ,连续观察术前 4周开始分别给予被动吸烟和腹腔注射尼古丁 (每次 0 .1mg/kg或 0 .4mg/kg,bid ,持续 6周 )对阿朴吗啡诱发的旋转行为、纹状体多巴胺 (dopamine,DA)的含量和黑质酪氨酸羟化酶 (TyrosineHydroxylase ,TH)阳性神经细胞数目的影响。结果 :被动吸烟和腹腔注射尼古丁的大鼠旋转行为明显减少 ,受损侧纹状体DA含量和黑质TH阳性神经元的数目较对照组增高 (P <0 .0 1) ,高剂量尼古丁作用更为显著。结论 :烟草成份可减轻 6 OHDA对黑质DA神经元的损伤
OBJECTIVE: To investigate the neurotoxic effect of tobacco components on the protection of dopamine neurons against 6-hydroxydopamine (6-OHDA). Methods: The rat model of Parkinson’s disease was established by stereotactic injection of 6 OHDA in rat brain. The rats were treated with passive smoking and intraperitoneal injection of nicotine (0.1 mg / kg or 0.4 mg / kg bid for 4 weeks) 6 weeks) on apomorphine-induced rotation, the content of dopamine (DA) and the number of tyrosine hydroxylase (TH) positive neurons in striatum. RESULTS: Passive smoking and intraperitoneal injection of nicotine reduced the rotational behavior of rats significantly. The DA content in substantia nigra striatum and the number of TH positive substantia nigra neurons were significantly higher than those in the control group (P <0.01), and high-dose nicotine More significant. Conclusion: Tobacco ingredients can reduce the damage of substantia nigra DA neurons by 6 OHDA