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[目的]探讨桦褐孔菌多糖对葡聚糖硫酸钠(DSS)诱导的急性炎症性肠病(IBD)模型小鼠的影响.[方法]取7周龄Balb/c雄性小鼠,随机分为正常对照组、模型对照组和实验组.每日灌胃给予实验组小鼠200mg/kg桦褐孔菌多糖,给模型对照组和实验组小鼠自由饮用3.5%(质量分数)DSS溶液7d,第8d饮用纯净水,正常对照组一直自由饮用纯净水.每天测量体质量,于实验第9d处死小鼠取结肠,测量结肠长度,并进行结肠黏膜大体形态学和组织学评分.用半定量RT-PCR法测定结肠组织和肠系膜淋巴结(MLN)中肿瘤坏死因子(TNF)-α和白细胞介素(IL)-17的表达水平.[结果]模型对照组和实验组体质量均下降,但与模型对照组比较,实验组小鼠体质量下降幅度减小,于实验第7~9 d,两组体质量下降幅度间差异有统计学意义(P<0.05).模型对照组结肠平均长度较正常对照组明显减少(P<0.01),而实验组结肠平均长度较模型对照组缩短程度明显减轻(P<0.05).实验组结肠大体和组织学评分均明显低于模型对照组(P<0.05),结肠组织和MLN中TNF-α和IL-17 mRNA表达均少于模型对照组.[结论]桦褐孔菌多糖对DSS诱导的急性IBD模型小鼠具有治疗作用,其机制认为可能与下调结肠组织和MLN中促炎细胞因子TNF-α和IL-17表达有关系.
[Objective] To investigate the effect of Inonotrichia polysaccharides on Dextran Sodium Sulfate (DSS) induced acute inflammatory bowel disease (IBD) model mice. [Methods] Male Balb / c 7 week old mice were randomly divided As control group, model control group and experimental group.The mice in experiment group were given intragastric administration of 200mg / kg polysaccharide of Inonotus obliquus, and the model control group and experimental group were given 3.5% (mass fraction) DSS solution freely for 7 days , Pure water was used on the 8th day, pure water was freely consumed in the normal control group, body weight was measured daily, and the colon was sacrificed on the 9th day after the experiment, the length of the colon was measured, and the morphology and histology of the colon mucosa were scored. The levels of tumor necrosis factor (TNF) -α and interleukin (IL) -17 in colonic tissue and mesenteric lymph nodes (MLN) were measured by RT-PCR. [Results] The body weight of model control group and experimental group decreased, Compared with the model control group, the decrease of body weight of mice in the experimental group decreased in the experimental group from the 7th to the 9th day (P <0.05), and the average length of the colon in the model control group was The normal control group was significantly reduced (P <0.01), while the experimental group, the average length of colon than the model (P <0.05) .The colon and histological scores of experimental group were significantly lower than those of model control group (P <0.05), while the expressions of TNF-α and IL-17 mRNA in colon tissue and MLN were less than those in model group Control group. [Conclusion] The polysaccharide of Inonotus obliquus has the therapeutic effect on DSS-induced acute IBD model mice, and the mechanism may be related to the down-regulation of the expression of pro-inflammatory cytokines TNF-α and IL-17 in colonic tissue and MLN.