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目的评价环卵沉淀试验(Circum oval precipitin test COPT)在广州管圆线虫病检测诊断中的应用价值。方法根据广州管圆线虫感染时间不同将SD大鼠分为B、C、D 3组,(A组为正常SD大鼠,E、F组分别为感染血吸虫、旋毛虫的SD大鼠),每组5张单凹载玻片,每张采用10个广州管圆线虫虫卵,与感染前后不同时间段大鼠血清各30μL进行反应,放置370C孵箱内孵育,72h后置显微镜下观察阳性反应结果并计算环沉率。结果广州管圆线虫幼虫感染后7d、14d和28d,SD大鼠血清环沉率分别为24%、68%、90%;感染前SD大鼠血清、血吸虫阳性血清和旋毛虫阳性血清广州管圆线虫环沉率分别为0%、0%和2%。结论 COPT法检测感染广州管圆线虫病的大鼠血清抗体具有一定的免疫学诊断价值,为广州管圆线虫病散发病例的快速诊断提供可选择的方法。
Objective To evaluate the value of Circum oval precipitin test (COPT) in the diagnosis of angiostrongychiasis in Guangzhou. Methods According to the different time of infection of C. elegans, SD rats were divided into three groups: group B, C and D (group A was normal SD rats, group E and F were infected with schistosome and trichinella respectively) Groups of 5 single concave slides, each with 10 of the Chinese worm worm eggs, and before and after infection at different time intervals rat serum 30μL reaction, placed 370C incubator incubated 72h after the microscope under the microscope to observe the positive reaction results Calculate the ring deposition rate. Results At 7, 14 and 28 days after infection, the serum levels of SD rats were 24%, 68% and 90%, respectively. Serum, Schistosoma japonicum positive serum and Trichinella spiralis serogroups Nematode loop rates were 0%, 0% and 2%, respectively. CONCLUSION: The COPT assay of serum antibodies against the infection of angiostrongylus gonorrhoeae has some immunological diagnostic value and provides an alternative method for the rapid diagnosis of angiostrongyliasis in Guangzhou.