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目的:观察胰岛素皮下(s.c.)、腹腔(i.p.)注射两种给药途径对2型糖尿病小鼠血糖的影响.方法:采用空腹血糖高于20.0 mmol/L的雄性Kk小鼠作为2型糖尿病模型,分为s.c.组(n=6)和i.p.组(n=6),分别s.c.和i.p.不同剂量的胰岛素,一次给药后尾静脉测血糖,连续监测2 h.结果:非禁食状态下,s.c.和i.p.组注射胰岛素前血糖分别为(32.0±1.5)、(31.0±1.5)mmol/L,注射胰岛素后血糖逐渐下降.胰岛素剂量不足时,血糖30 min时达最低点,随后逐渐上升;胰岛素适量时,血糖30 min时达正常水平,维持一定时间后逐渐上升;胰岛素剂量过高时,血糖30 min时低于正常水平,并且持续缓慢降低.以30 min时的血糖值为药效指标,做剂量-效应关系曲线,胰岛素剂量在1.1～1.7 U/kg范围时,i.p.组血糖可稳定地控制在正常水平(5.1±0.2)mmol/L,曲线形成显著的平台;s.c.组的血糖则在2.1～9.2 mmol/L之间,与剂量-效应曲线呈线性关系.对于本组实验,腹腔胰岛素给药的有效剂量范围为皮下给药最适剂量的±21%.结论:胰岛素腹腔给药较皮下给药控制血糖更为平稳,血糖波动小,不易造成低血糖.“,”AIM: To observe the effects of subcutaneous and intraperitoneal injection for insulin on the rats with Type II diabetes. METHODS: Kk mice with fasting blood sugar level higher than 20 mmol/L was set as Type II diabetes model and divided into subcutaneous injection group ( s. C. Group, n = 6) and intraperitoneal injection group ( I. P. Group, n = 6 ) with different dosis of insulin, and the tail blood sugar levels were monitored for two continuous hours after administration. RESULTS: Under non-fasting condition, the blood sugar levels of s. C. Group and I. P. Group were (32.0± 1.5), (31.0 ± 1.5) mmol/L,respectively before insulin injection, and were decreased gradually after injecting. When insulin dose was not sufficient, the blood sugar level was at the bottom at the 30th minutes , and then went upwards gradually. When the dose was appropriate, the blood sugar level was at normal level at the 30th minutes, and then went upwards gradually after maintaining a certain time. When insulin wasrnoverdose, the blood sugar level was below normal level and decreased gradually and slowly. The dose-response curve was drawn according to the blood sugar level at the point of 30 minutes. When the insulin dose was in the range of 1.1 - 1.7 U/kg, the blood sugar level could be stably controlled within normal scope (5.1 ± 0.2) mmol/L in I.p. Group, and the dose-response curve showed an obvious plateau phase. The dose-response curve showed a linear relation with the blood sugar level in the range of 2.1 -9.2 mmol/L in s. C. Group. The effective dose scope of insulin intraperitoneal injection was ± 21 % of the subcutaneous injection dose. CONCLUSION: Compared with subcutaneous injection, ntraperitoneal injection can control blood sugar level more stably, cause less fluctuation and a lower chance of hypoglycemia.