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目的:观察姜黄素对小鼠肝癌前病变的预防作用。方法:每周腹腔注射二乙基亚硝胺(DEN,95 mg.kg-1)建立小鼠肝癌前病变,每天灌胃姜黄素(120 mg.kg-1)或姜黄素与山药(3.03 g.kg-1)联合用药,连续10周(70 d);第71天眼球后静脉丛取血,检测血清肝功指标丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和谷胱甘肽-S-转移酶(GST),肝脏经灌注固定后用光学显微镜观察病理组织学变化。结果:模型组小鼠肝脏同时存在变质与修复性病变,增生肝细胞具有异型性;血清ALT:(195.57±91.56)U.L-1、AST:(39.68±24.20)U.L-1、GST:(42.31±8.19)U.L-1升高明显(P<0.01)。姜黄素组肝损伤较轻,增生肝细胞异型性降低,ALT:(98.54±48.07)U.L-1、AST:(26.89±7.51)U.L-1、GST:(47.47±8.54)U.L-1下降明显(P<0.05),姜黄素联合山药组ALT:(82.75±38.54)U.L-1、AST:(30.32±7.80)U.L-1、GST:(24.61±7.58)U.L-1疗效优于单用(P<0.01)。结论:姜黄素对诱导性小鼠肝癌前病变具有防治作用,姜黄素与山药联合用疗效更佳。
Objective: To observe the preventive effect of curcumin on precancerous lesions of liver cancer in mice. Methods: The precancerous lesion of mouse hepatocarcinoma was induced by intraperitoneal injection of diethylnitrosamine (DEN, 95 mg.kg-1) every day. The rats were given intragastrically with curcumin (120 mg.kg-1) .kg-1) for 10 consecutive weeks (70 days); on the 71st day, the blood was taken from the retrobulbar venous plexus to detect the serum liver markers of alanine aminotransferase (ALT), aspartate aminotransferase ) And glutathione S-transferase (GST). The liver was observed for pathological changes by light microscopy after perfusion fixation. Results: The liver of model group had atypia and reparative lesion at the same time, and the liver cells were characterized by atypia. The levels of serum ALT: UL-1, AST: (39.68 ± 24.20) UL-1 and GST: (42.31 ± 8.19) UL-1 increased significantly (P <0.01). Curcumin group had less liver injury and reduced atypia of hyperplastic liver cells. ALT: UL-1, AST: (26.89 ± 7.51) UL-1 and GST: (47.47 ± 8.54) (82.75 ± 38.54) UL-1, AST: (30.32 ± 7.80) UL-1 and GST: (24.61 ± 7.58) UL-1 in curcumin group and yam group were better than those in single group (P < 0.01). Conclusion: Curcumin has a preventive and therapeutic effect on the precancerous lesions of hepatocarcinoma in mice, and the combination of curcumin and yam is better.