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[Objective] To compare the content changes of strychnine and brucine in Renqingmangjue combined with or without Zuota.[Methods]UPLC was used to determine the content. Chromatography conditions: Waters Acquity UPLC BEH C18column( 2. 1 mm × 50mm,1. 7 μm); mobile phase: composed of methanol and 0. 02% phosphoric acid( 12∶88,V / V); determine wavelength 260 nm; flow: 0. 4mL /min; column temperature: 40 ℃. [Results] The linear range of strychnine was 0. 004 16 to 0. 041 6 μg / mL,the average recovery rate was 100. 43% and the RSD was 1. 61%; The linear range of brucine was 0. 002 08- 0. 020 8 μg / mL,the average recovery rate was 100. 70%and the RSD was 1. 66%; the content of strychnine and brucine in Renqingmangjue pill combined with Zuota lowered 0. 91% and 8. 52% respectively. [Conclusions]The content of strychnine and brucine in Renqingmangjue pill combined with Zuota lowered at different degree and the content of brucine lowered more significantly which indicated that Renqingmangjue pill combined with Zuota was more efficiency and could reduce poisonousness.
[Objective] To compare the content changes of strychnine and brucine in Renqingmangjue combined with or without Zuota. [Methods] UPLC was used to determine the content. Chromatography conditions: Waters Acquity UPLC BEH C18column (2.1 mm × 50 mm, 1.7 μm); mobile phase: composed of methanol and 0.02% phosphoric acid (12:88, V / V); determine wavelength 260 nm; flow: 0. 4 mL / The range of strychnine was 0. 004 16 to 0. 041 6 μg / mL, the average recovery rate was 100. 43% and the RSD was 1. 61%; The linear range of brucine was 0. 002 08- 0. 020 8 μg / mL, the average recovery rate was 100. 70% and the RSD was 1. 66%; the content of strychnine and brucine in Renqingmangjue pill combined with Zuota lowered 0. 91% and 8. 52% respectively. [Conclusions] The content of strychnine and brucine in Renqingmangjue pill combined with Zuota lowered at different degree and the content of brucine lowered more significantly which indicated that Renqingma ngjue pill combined with Zuota was more efficiency and could reduce poisonousness.