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为了给甜樱桃微生物肥料的研制和生产提供优良菌株资源,本文从马哈利樱桃根际土壤中分离获得52株细菌,通过保绿法和萝卜子叶增重法进行初筛,筛选具有促生作用的菌株,进一步进行IAA产生菌的筛选,并经形态观察、生理生化特征及16SrDNA序列分析明确分类地位,利用分光光度法测定产IAA的能力,并对最佳碳氮源、产素条件进行了筛选。结果表明,52株菌中B20产IAA能力最强,经鉴定为阿氏芽孢杆菌(Bacillus aryabhattai),其最佳产素的培养条件为:采用30%的装液量在温度为30℃、转速为150r·min-1的条件下培养44h,最佳碳、氮源分别为果糖和酵母粉。研究发现菌株B20具有IAA产生能力,具有作为功能微生物肥料研发的潜力,可进一步对其进行开发研究。
In order to provide good strain resources for the research and production of sweet cherry microbial fertilizer, 52 strains of bacteria were isolated from Mahali cherry rhizosphere soils. The preliminary screening was carried out by green-keeping method and radish cotyledon weight gain method. The strains of IAA were further screened. The morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis were used to identify the strains. Spectrophotometry was used to determine the IAA production ability. The optimum conditions of carbon and nitrogen sources, filter. The results showed that 52 strains had the highest ability of producing IAA, which was identified as Bacillus aryabhattai. The optimal conditions for the production of Bovine serum were as follows: 30% Under the conditions of 150r · min-1 for 44h, the optimum carbon and nitrogen sources were fructose and yeast extract respectively. It has been found that strain B20 has the potential of IAA production and development as a functional microbial fertilizer and can be further developed.