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孕妇尿经酸水解、醚萃取、弱碱洗涤后用高效液相色谱法测定其中雌三醇的含量。雌三醇标准液在日内（ｎ＝５）和日间（ｎ＝５）ＲＳＤ分别为２．０５％和３．７２％。样品平均回收率为８５．３９％，ＲＳＤ为２．２７％。此法快速准确，已用于产科的临床分析。
Uterine urine by acid hydrolysis, ether extraction, washing with weak base after the determination of the contents of estriol by high performance liquid chromatography. The estriol standards showed 2.05% and 3.72% RSDs during the day (n = 5) and during the day (n = 5), respectively. The average recovery of samples was 85.39% with RSD of 2.27%. This method is fast and accurate and has been used in clinical analysis of obstetrics.