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试验采用营养液培养法,研究酸铝胁迫对不同柱花草品种幼苗根系生长和生理性状的影响,比较分析不同品种间的反应差异,并结合土壤盆栽方法进行验证。结果表明:1、用低浓度铝溶液浸泡柱花草种子,种子相对发芽率和幼苗根系生长受到明显抑制,在10μm o l.L-1、30μm o l.L-1和50μm o l.L-1铝浓度胁迫下,热研10号的相对发芽率比其他5个品种都高,而热研13号均为最低;2、在30μm o l.L-1铝溶液胁迫下,幼苗种根相对伸长率受到显著抑制,根尖苏木精染色指数增加,热研10号受抑制最小且根尖着色程度最低,而热研7号和热研13号表现受抑制最大及着色程度最高;3、用30μm o l.L-1铝溶液胁迫处理柱花草幼苗,幼苗根系活跃吸收面积降低,细胞质膜透性和根系活力上升;10d后热研10号的根系活跃吸收面积近4倍大于热研13号;质膜透性及根系活力的变化也显示出不同柱花草品种间的耐酸铝性存在显著差异,热研10号对铝耐受性最强,而热研7号及热研13号对铝最敏感;热研5号、C IAT 184和907的对酸铝胁迫的反应介于两者之间。
The nutrient solution culture method was used to study the effects of acid-aluminum stress on the growth and physiological traits of roots of different stem flower seedlings. The differences among different varieties were compared and analyzed, and the soil potted plants were used for validation. The results showed that: 1, the seeds of Prunus mumewere soaked in low concentration of aluminum solution, the relative germination rate of seed and seedling root growth were significantly inhibited. Under aluminum stress of 10μm o lL-1, 30μm oLL-1 and 50μm oLL-1, The relative germination rate of Zheyan 10 was higher than that of the other 5 cultivars, while that of Zheyan 13 was the lowest.2. Under the stress of 30μm o LL-1 aluminum solution, the relative elongation of seedling roots was significantly inhibited, The index of hematoxylin staining increased, the number of hot research No. 10 was the least and the color of the root tip was the lowest, while that of No.7 and No.13 was the highest and the degree of coloring was the highest.3. With 30μm o lL-1 aluminum After treated with solution, the seedling of Prunus mongolica was reduced, the active absorption area of seedling roots decreased, and the permeability of plasma membrane and root activity increased. After 10 days, the root active absorption area of Gerian 10 was nearly 4 times larger than that of Gerian 13. The membrane permeability and root activity The results also show that there are significant differences in the acid resistance of aluminum between different column flower varieties. The thermal resistance of hot research 10 is the strongest to aluminum, while that of hot research 7 and hot research 13 is the most sensitive to aluminum. Hot research 5, The reaction of C IAT 184 and 907 to aluminum and aluminum stress is in-between.