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目的:探讨儿童急性白血病流式细胞术免疫分型的意义。方法:采用流式细胞术三色荧光标记技术和CD45/SSC双参数散点图设门,检测185例儿童急性白血病的免疫表型,对抗原表达情况进行分析。结果:流式细胞术免疫分型和FAB分型的符合率为89.19%。185例儿童急性白血病中,ALL为121例,占AL的65.41%,B-ALL为113例,主要表达B系的CD19(99.12%)、CD22(98.13%)、CD79a(96.19%)、CD10(86.73%)。T-ALL占8例;主要表达CD5(100%)、CD7(100%)、cCD3(100%)、CD8(87.5%)。AML为47例,占25.41%,主要表达CD33(93.62%)、CD15(78.72%)、CD64(76.6%)、MPO(76.6%)、CD13(74.47%)。在B-ALL,AML,T-ALL中,敏感性最高的抗体分别是CD19,CD33,CD5和CD7,特异性最强的抗体分别是CD79a,MPO,cCD3。AMLL为17例,占9.19%,其中B/M为9例,T/B为5例,T/M为3例。My十-ALL为54例,占ALL的44.63%,表达的髓系抗原为CD13、CD15、CD33、CD64。Ly+-AML为18例,占AML的38.30%,表达的淋系抗原为CD19、CD4、CD7。系列非相关抗原CD34的表达率为67.57%,HLA-DR的表达率为85.41%,CD38的表达率为80.59%,TdT的表达率为62.59%。结论:流式细胞术免疫分型在白血病分型中起重要作用,是FAB分型的补充和修正,提高了儿童急性白血病诊断的准确率有必要进一步加强流式细胞术免疫分型的标准化工作。
Objective: To investigate the significance of immunophenotyping in childhood acute leukemia by flow cytometry. Methods: The immunophenotypes of 185 children with acute leukemia were detected by flow cytometry with three-color fluorescence labeling and CD45 / SSC double-parameter scatter plot. The antigen expression was analyzed. Results: The coincidence rate of FACS and immunofluorescence was 89.19%. In 185 children with acute leukemia, ALL was 121 (65.41%) and B-ALL was 113 (99.12%), CD22 (98.13%), CD79a (96.19%) and CD10 ( 86.73%). T-ALL accounted for 8 cases; mainly expressed CD5 (100%), CD7 (100%), cCD3 (100%), CD8 (87.5%). There were 47 cases (25.41%) with AML, the main expressed CD33 (93.62%), CD15 (78.72%), CD64 (76.6%), MPO (76.6%) and CD13 (74.47%). Among B-ALL, AML and T-ALL, the most sensitive antibodies were CD19, CD33, CD5 and CD7, respectively. The most specific antibodies were CD79a, MPO and cCD3, respectively. AMLL was 17 cases, accounting for 9.19%, of which B / M was 9 cases, T / B was 5 cases, T / M was 3 cases. My -ALL was 54 cases, accounting for 44.63% of ALL. The expressed myeloid antigens were CD13, CD15, CD33 and CD64. Ly + -AML was 18 cases, accounting for 38.30% of AML, the expression of lymphoid antigen CD19, CD4, CD7. The expression of CD34 was 67.57%, the expression of HLA-DR was 85.41%, the expression of CD38 was 80.59% and the expression of TdT was 62.59%. CONCLUSION: Flow cytometry immunophenotyping plays an important role in the typing of leukemia, which is the complement and amendment of FAB typing and improves the diagnostic accuracy of acute leukemia in children. It is necessary to further standardize the immunofluorescence of flow cytometry .