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本实验从70名学生中收集了100个集合菌斑,其中有龋者(DMFT3～8)和无龋者各35人。样品的一半为“自然”菌斑,另一半是用各种食用糖漱口后收集的。分析结果表明,漱糖前菌斑EPS含量平均为菌斑干重的9.10％,水溶性和碱溶性EPS的分布比例接近70:30。患龋与无龋者菌斑EPS的含量(分别为9.16％和9.05％),和水、碱溶性EPS的分布均无显著性差异(P>0.5)。用蔗糖与麦芽糖漱口后EPS分别比漱糖前增加了46.20％和49.47％,其差值均有高度显著性(P<0.001)。同时还发现这二种糖能显著改变水、碱溶性多糖的分布比例。但含漱葡萄糖、果糖和木糖醇后,菌斑EPS的含量与分布均无明显变化。
In this study, 100 collection plaques were collected from 70 students, including caries (DMFT3 ~ 8) and caries-free 35 people each. Half of the samples were “natural” plaque and the other half was collected after gargling with various edible sugars. The results showed that the average EPS content of swamp sugar plaque was 9.10% of the dry weight of the plaque, and the proportion of water-soluble and alkali-soluble EPS was nearly 70:30. There was no significant difference (P> 0.5) in the distribution of EPS in caries-bearing and caries-free plaque (9.16% and 9.05%, respectively). After gargling with sucrose and maltose, EPS was increased by 46.20% and 49.47%, respectively, compared with before gargle, the difference was highly significant (P <0.001). Also found that these two kinds of sugar can significantly change the water, alkali-soluble polysaccharide distribution ratio. However, no significant changes were found in the content and distribution of plaque EPS after gargle with glucose, fructose and xylitol.