论文部分内容阅读
目的 建立人体包虫原头节的体外培养模型 ,评价抗棘球蚴人源单链可变区抗体(ScFv)抑制人体包虫原头节的效果。方法 无菌抽取人体包虫囊肿原头节 ,与ScFv高 (1mg/L)、中(0 .1mg/L)、低 (0 .0 1mg/L)剂量组和对照组 (等量的培养液 )共同培养 ,比较各组原头节死亡率和残余ScFv浓度 ,观察原头节形态学改变。结果 高剂量ScFv组原头节死亡率与对照组的差异均有统计学意义 (P <0 .0 1) ,但中、低剂量组与对照组死亡率的差异无统计学意义 (P >0 .0 5 ) ,随作用天数增加 ,高剂量ScFv组原头节结构逐渐被破坏、皱缩、崩解。结论 该ScFv具备体外抑制人体原头节的作用 ,其抑制作用与剂量和时间有关。
OBJECTIVE: To establish an in vitro model of human eutaci suturalis and evaluate the effect of human single chain variable region antibody against human hydatid cyst (ScFv) Methods Aneurysm of the human hydatid cyst was extracted aseptically and compared with the ScFv high (1 mg / L), moderate (0.1 mg / L) and low (0.01 mg / L) ) Were co-cultured. The mortality and residual ScFv concentration in each group were compared, and morphological changes of protoscoleces were observed. Results The mortality in the high dose ScFv group was significantly lower than that in the control group (P <0.01), but there was no significant difference in mortality between the medium and low dose groups and the control group (P> 0) .0 5). With the increase of days, the structure of the protoscoleces in the high-dose ScFv group was gradually destroyed, collapsed and disintegrated. Conclusion The ScFv has the effect of inhibiting the proroca in vitro and its inhibitory effect is related to the dose and time.