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目的 :研究G1 →S调控点中细胞周期数D1(cyclinD1)、p16及视网膜母细胞瘤蛋白 ( pRB)基因在胶质瘤中表达情况 ,分析与细胞增殖和凋亡的关系 ,探讨肿瘤发生的原因。方法 :37例人脑胶质瘤标本按WHO分类标准 ( 1990 )分为 :星形细胞瘤 2 5例 (纤维型 7例 ,原浆型 6例 ,间变型 12例 ) ,胶质母细胞瘤 12例 (包括GBM 4例 )。正常对照脑组织 10例。cyclinD1 、p16、pRB和Ki 6 7的表达用免疫组化的方法 ,凋亡细胞通过TUNEL进行检测 ,细胞增殖和凋亡的评估分别用Ki 6 7LI和凋亡指数 (AI)。结果 :三种因子在胶质瘤中都存在着异常 ,其中cyclinD1表现为过度表达 ( 2 9 37,78.4%) ,p16、pRB表现为缺失 ( 2 1 37,5 6 .8%和 13 37,35 .1%) ,且都与肿瘤分型有关 ,在恶性肿瘤中更加明显 ;pRB路径的异常在胶质瘤中更为频发 ,与肿瘤恶化有关 ;pRB路径异常时 ,Ki 6 7LI和AI均明显增高 ;在星形细胞的肿瘤中 ,凋亡发生比较少见 (星形细胞瘤 :0 .0 10± 0 .0 0 2 ;胶质母细胞瘤 :0 .0 5 7± 0 .0 16 ) ,与肿瘤分型有关。结论 :cyclinD1 p16 pRB路径的异常与胶质瘤的发生和生长关系密切。
OBJECTIVE: To study the expression of cyclinD1, p16 and pRB gene in G1 → S regulatory sites in gliomas, and to investigate the relationship between the expression of cyclin D1, p16 and proliferation and apoptosis in gliomas, the reason. Methods: Thirty-seven human gliomas were divided into two groups according to the WHO classification criteria (1990): 25 cases of astrocytoma (7 cases of fibroids, 6 cases of primordial plasma, 12 cases of metaplasia), glioblastoma 12 cases (including 4 cases of GBM). Normal control brain tissue in 10 cases. The expression of cyclinD1, p16, pRB and Ki 6 7 were detected by immunohistochemistry. Apoptotic cells were detected by TUNEL. The cell proliferation and apoptosis were evaluated by Ki67L and AI respectively. Results: There were abnormalities of all three factors in gliomas, including cyclinD1 overexpression (2 37.78%), p16 pRB deletion (2137 56.8% and 13 37% 35.1%), all of which were related to tumor typing and were more obvious in malignant tumors. The abnormalities of pRB pathway were more frequent in glioma and were related to tumor progression. In abnormal pathways of pRB, Ki 6 7LI and AI Were significantly higher in the astrocytic tumors, the occurrence of apoptosis is relatively rare (astrocytoma: 0.010 ± 0.002; glioblastoma: 0.07 ± 0. 0 16 ), And tumor type related. Conclusion: Abnormality of cyclinD1 p16 pRB pathway is closely related to the occurrence and growth of glioma.