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目的 探讨运动对高脂饮食老年大鼠血脂代谢的影响。 方法 建立饮食性高脂血症大鼠模型 ,采用不同时间 (45min和 90min)的游泳运动 ,测定各组大鼠血清胆固醇 (TC)、血清高密度脂蛋白胆固醇 (HDL C)和载脂蛋白A1(apoA1) ;并用逆转录聚合酶链式反应 (RT PCR)检测各组大鼠肝脏apoA1mRNA水平。 结果 参加运动的高脂饮食大鼠血清HDL C分别为 (1 4 8± 0 2 4 )mmol/L和(1 4 9± 0 2 5 )mmol/L ,apoA1分别为 (0 13± 0 0 7)mmol/L和 (0 17± 0 0 3)mmol/L ,均较对照组和单纯高脂组升高 (P <0 0 5 ) ,高脂饮食的老年大鼠肝脏apoA1mRNA表达明显减少为 0 6 5 1± 0 2 4 (P <0 0 1) ,而游泳运动则使其显著恢复 ,两运动组apoA1mRNA表达量分别为 1 6 2 3± 0 6 1和 1 5 77± 0 4 9,差异无显著性 (P <0 0 5 )。 结论 游泳运动通过调节大鼠肝脏apoA1mRNA的表达 ,影响高脂饮食大鼠血浆高密度脂蛋白 (HDL)的变化。
Objective To investigate the effect of exercise on lipid metabolism in aged rats with high fat diet. Methods The rat model of diet-induced hyperlipidemia was established. The swimming exercise at different times (45min and 90min) was used to measure the levels of serum cholesterol (TC), serum high density lipoprotein cholesterol (HDL C) and apolipoprotein A1 (apoA1). The level of apoA1 mRNA in liver of each group was detected by reverse transcription polymerase chain reaction (RT PCR). Results Serum HDL C of high fat diet rats participating in exercise was (144 ± 024) mmol / L and (149 ± 025) mmol / L respectively, while apoA1 was (0 13 ± 0 0 7 ) mmol / L and (0 17 ± 0 0 3) mmol / L, respectively, compared with the control group and the simple hyperlipidemia group (P <0.05). The expression of apoA1mRNA in the liver of high- 6 5 1 ± 0 2 4 (P 0 01), while the swimming exercise significantly restored the expression of apoA1 mRNA in the two exercise groups, which was respectively 1 6 2 3 ± 0 6 1 and 1 5 77 ± 0 49 No significant (P <0 05). Conclusion Swimming can affect the changes of plasma high density lipoprotein (HDL) in high fat diet rats by regulating the expression of apoA1 mRNA in rat liver.