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探讨肿瘤坏死因子(TNF-α)在人膜性肾小球肾炎中的定量表达。采用免疫组织化学SABC法检测20例人的膜性肾小球肾炎穿刺标本中TNF-α的表达,并用计算机图像分析系统进行定量分析。发现TNF-α蛋白在肾组织内表达增加,阳性反应见于肾小管上皮细胞和肾小球内,以肾近曲小管上皮细胞阳性最明显,肾小球内呈程度不等的弱阳性,间质反应不明显。肾炎组肾小管阳性表达与正常组相比差异有显著性(p<0.01),表明TNF-α在肾小管的损伤和肾间质纤维化中起重要的介质作用。
To investigate the quantitative expression of tumor necrosis factor (TNF-α) in human membranous glomerulonephritis. Immunohistochemical SABC method was used to detect the expression of TNF-α in 20 cases of membranous glomerulonephritis puncture specimens and quantified by computer image analysis system. Found that TNF-α protein expression increased in renal tissue, the positive reaction found in renal tubular epithelial cells and glomerular, renal proximal tubule epithelial cells were most obvious, glomerular within a certain degree of weak positive, interstitial The reaction is not obvious. Nephritis group renal tubule expression was significantly different from the normal group (p <0.01), indicating that TNF-α plays an important mediator in renal tubular injury and renal interstitial fibrosis.