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类黄酮3-羟化酶是类黄酮生物合成途径中的关键酶,对花色的形成起重要作用。本研究克隆了鹤望兰类黄酮3-羟化酶基因SrF3H,该cDNA全长1 333 bp(GenBank收录号:KC412257),具有完整的开放阅读框(ORF),共1 122个碱基,编码374个氨基酸。与其他物种的氨基酸序列都有一定的同源性,其中与高杯花的F3H同源性最高,达到83%。系统进化树分析显示,鹤望兰SrF3H与百合蛋白亲缘关系较近。应用半定量PCR分析表明,SrF3H在蓝色花瓣中表达最高,而在鹤望兰不同花发育阶段差异不明显。研究结果不仅为植物育种提供了重要基因资源,也为剖析鹤望兰花色形成机理奠定基础。
Flavonoids 3-hydroxylase is the key enzyme in flavonoid biosynthesis pathway, which plays an important role in the formation of flower color. This study cloned the strelitzin flavonoid 3-hydroxylase gene SrF3H, the full-length cDNA of 1 375 bp (GenBank accession number: KC412257), with a complete open reading frame (ORF) of 1 122 bases encoding 374 amino acids. It shared some homology with the other species’ amino acid sequences, and the homology with F3H was 83%. Phylogenetic tree analysis showed that the genetic relationship between Strelitzia reginae SrF3H and lily protein was close. Semi-quantitative PCR analysis showed that SrF3H had the highest expression in blue petals, but not in the different flower development stages of Strelitzia reginae. The results not only provide important gene resources for plant breeding, but also lay a foundation for analyzing the formation mechanism of flower color of Strelitzia reginae.